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	Immunolocalization of matrix proteins in nacre lamellae and their in vivo effects on aragonitic tablet growth
	Gong, Ningping ; Shangguan, Junlong ; Liu, Xiaojun ; Yan, Zhenguang ; Ma, Zhuojun ; Xie, Liping ; Zhang, Rongqing
	2010-05-11 ; 2010-05-11 ; OCT
关键词	Mollusk shell nacre matrix protein BMP-2 nacrein antibodies CARBONIC-ANHYDRASE ORGANIC MATRIX PINCTADA-FUCATA SHEET NACRE BONE BIOMINERALIZATION CELLS CALCIFICATION OSTEOBLASTS OYSTER Biochemistry & Molecular Biology Biophysics Cell Biology
中文摘要	How matrix proteins precisely control the growth of nacre lamellae is an open question in biomineralization research. Using the antibodies against matrix proteins for immunolabeling and in vivo experiments. we investigate the structural and functional roles of EDTA-soluble matrix (SM) and EDTA-insoluable matrix (ISM) proteins in nacre biomineralization of the pearl oyster Pinctada fucata. Immunolabeling reveals that a SM proteins, nacrein, distributes within aragonitic tablets and intertabular matrix. An ISM protein. Which we named P43, has been specifically recognized by polyclonal antibodies raised against the recombinant protein of P.fucata bone morphogenetic protein 2 in immunoblot analysis. Immunolabeling indicates that P43 is localized to interlamellar sheet and also embedded within aragonitic tablets. Although nacrein and P43 both distribute within aragnoitic tablets, they function differently in aragonitic tablet growth. When nacrein in suppressed by the antibodies agaisnt it in vivo, crystal over-growth occurs, indicating that this SM protein is a negative regulator in aragonitic tablet growth. When P43 is suppressed in vivo, the organo-mineral assemblage is distrupted, suggesting that P43 is a frame-work matrix. Taken together, SM and ISM proteins are indispensable factors for the growth of nacre lamellae, controlling crystal growth and constructing and the framework of aragonitic tablets. (c) 2008 Elsevier Inc. All rights reserved.
语种	英语 ; 英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE ; SAN DIEGO ; 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26990]
专题	清华大学
推荐引用方式 GB/T 7714	Gong, Ningping,Shangguan, Junlong,Liu, Xiaojun,et al. Immunolocalization of matrix proteins in nacre lamellae and their in vivo effects on aragonitic tablet growth[J],2010, 2010, OCT.
APA	Gong, Ningping.,Shangguan, Junlong.,Liu, Xiaojun.,Yan, Zhenguang.,Ma, Zhuojun.,...&Zhang, Rongqing.(2010).Immunolocalization of matrix proteins in nacre lamellae and their in vivo effects on aragonitic tablet growth..
MLA	Gong, Ningping,et al."Immunolocalization of matrix proteins in nacre lamellae and their in vivo effects on aragonitic tablet growth".(2010).

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