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	Isolation of a cotton reversibty glycosylated polypeptide (GhRGP1) promoter and its expression activity in transgenic tobacco
	Wu, AM ; Ling, C ; Liu, JY
	2010-05-11 ; 2010-05-11
关键词	cotton promoter activity reversibly glycosylated polypeptide transgenic tobacco wound-induced expression CELL-WALL BIOSYNTHESIS EXTA EXTENSIN GENE PROTEIN GENE MOLECULAR-CLONING FIBER DEVELOPMENT LOW-TEMPERATURE UDP-GLUCOSE PLANT TRANSCRIPTION ARABIDOPSIS Plant Sciences
中文摘要	Reversibly glycosylated polypeptides (RGPs) are thought to be involved in polysaccharide metabolism. A cDNA of the cotton (Gossypium hirsutum) RGP gene, designated GhRGP1, has previously been characterized, and is preferentially expressed in fiber cells. In order to investigate its temporal and spatial control, we isolated a 624bp fragment upstream of the GhRGP1 coding sequence using a polymerase chain reaction (PCR)-based genomic walking method, transcriptionally fused the 624bp promoter sequence to the beta-glucuronidase (GUS) gene, and analyzed the stable gene expression in tobacco (Nicotiana tabacum). In 4-week-old transgenic tobacco plants, the highest expression level was observed in roots, and the GUS activity was 1.13- and 6.65-fold higher than that in stems and leaves, respectively. In the reproductive growth stage, the GUS expression level was highest in the pistils and the GUS activity in the stigmas and styles were 17.6-fotd higher than that in the ovaries. High GUS activity was also detected in the anthers. In addition, histochemical staining for GUS activity on transgenic tobacco plants further indicated a higher expression in the trichomes, seeds and vascular tissues of stems. Abiotic stress treatments on transgenic tobacco plants showed that wounding and dehydration induced GUS expression. These results demonstrated the spatial and temporal regulation of a cotton RGP promoter in a model plant, and provided an important insight into the factors that control the fiber development and stress responses of the gene. (c) 2005 Elsevier GmbH. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER GMBH, URBAN & FISCHER VERLAG ; JENA ; OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26835]
专题	清华大学
推荐引用方式 GB/T 7714	Wu, AM,Ling, C,Liu, JY. Isolation of a cotton reversibty glycosylated polypeptide (GhRGP1) promoter and its expression activity in transgenic tobacco[J],2010, 2010.
APA	Wu, AM,Ling, C,&Liu, JY.(2010).Isolation of a cotton reversibty glycosylated polypeptide (GhRGP1) promoter and its expression activity in transgenic tobacco..
MLA	Wu, AM,et al."Isolation of a cotton reversibty glycosylated polypeptide (GhRGP1) promoter and its expression activity in transgenic tobacco".(2010).

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