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	Trichosanthin's interfacial interactions with phospholipids: a monolayer study
	Xia, XF ; Wang, F ; Yang, MS ; Sui, SF
	2010-05-11 ; 2010-05-11
关键词	lipid/protein interaction air/water interface pi-A isotherm epifluorescence microscopy FLUORESCENCE MICROSCOPY PHOSPHATIDYLCHOLINE MONOLAYERS DIPHTHERIA-TOXIN HIV DISEASE SP-B PROTEIN PHASE BINDING FILMS PH Biophysics Chemistry, Physical Materials Science, Biomaterials
中文摘要	Lipid monolayer at the air/water interface, as half a membrane, was used here to investigate the interaction between trichosanthin (TCS), a ribosome inactivating protein, and phospholipid membrane. First, the protein adsorption experiments showed that the negatively charged DPPG caused obvious enrichment of TCS beneath the monolayer, indicating electrostatic attraction between TCS and the negatively charged phospholipid. Second, when TCS was incorporated into the phospholipid monolayer, it could not be completely squeezed out until the monolayer collapsed. The results were demonstrated to be irrelative with the phospholipid headgroup, suggesting a strong hydrophobic force between TCS and phospholipid hydrocarbon chain was involved in the interaction. Third, the protein/membrane interaction was further studied with fluorescence microscope. The results showed that TCS could penetrate into both the condensed and the fluid phase of the DPPG monolayer under low pH condition and eventually resulted in a homogeneous phospholipid phase. The breakage of ordered packing of phospholipid by TCS may be responsible for this homogenizing effect. (C) 2004 Elsevier B.V. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCIENCE BV ; AMSTERDAM ; PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26796]
专题	清华大学
推荐引用方式 GB/T 7714	Xia, XF,Wang, F,Yang, MS,et al. Trichosanthin's interfacial interactions with phospholipids: a monolayer study[J],2010, 2010.
APA	Xia, XF,Wang, F,Yang, MS,&Sui, SF.(2010).Trichosanthin's interfacial interactions with phospholipids: a monolayer study..
MLA	Xia, XF,et al."Trichosanthin's interfacial interactions with phospholipids: a monolayer study".(2010).

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