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	Membrane dielectric responses of bufalin-induced apoptosis in HL-60 cells detected by an electrorotation chip
	Huang, Chengjun ; Chen, Ailiang ; Guo, Min ; Yu, Jun
	2010-05-11 ; 2010-05-11
关键词	apoptosis electrorotation membrane capacitance membrane conductance HUMAN LEUKEMIA-CELLS DIELECTROPHORETIC FORCE CANCER CELLS EXPRESSION GENES Biotechnology & Applied Microbiology
中文摘要	A non-invasive electrorotation (ROT) technique was used to monitor the apoptosis-induced changes in HL-60 cells. The specific membrane capacitance of the cells fell from 15.6 +/- 0.9 mF/cm(2) stop to 6.4 +/- 0.6 mF/cm(2) stop after 48 h treatment with 10 nM bufalin, a component of bufadienolides in traditional Chinese medicine, Chan Su. However, the average membrane conductance remained almost constant during the first 24 h of treatment and then increased afterwards. Apoptosis was verified by a DNA fragmentation assay and scanning electron microscopy. The results demonstrate that the ROT technique gives a quantitative analysis of the toxic damage by chemicals to cells and can be exploited in the testing and development of new pharmaceuticals and active cell agents.
语种	英语 ; 英语
出版者	SPRINGER ; DORDRECHT ; VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26514]
专题	清华大学
推荐引用方式 GB/T 7714	Huang, Chengjun,Chen, Ailiang,Guo, Min,et al. Membrane dielectric responses of bufalin-induced apoptosis in HL-60 cells detected by an electrorotation chip[J],2010, 2010.
APA	Huang, Chengjun,Chen, Ailiang,Guo, Min,&Yu, Jun.(2010).Membrane dielectric responses of bufalin-induced apoptosis in HL-60 cells detected by an electrorotation chip..
MLA	Huang, Chengjun,et al."Membrane dielectric responses of bufalin-induced apoptosis in HL-60 cells detected by an electrorotation chip".(2010).

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