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	Simultaneous measurement of trace monoadenosine and diadenosine monophosphate in biomimicking prebiotic synthesis using high-performance liquid chromatography with ultraviolet detection and electrospray ionization mass spectrometry characterization
	Liu, H ; Zhao, C ; Lu, JS ; Liu, M ; Zhang, SS ; Jiang, YY ; Zhao, YF
	2010-05-11 ; 2010-05-11
关键词	N-phosphoryl amino acid adenosine adenosine monophosphate diadenosine monophosphate high-performance liquid chromatography electrospray ionization mass spectrometry ADENOSINE NUCLEOTIDES AMINO-ACIDS NUCLEOSIDES PHOSPHOTHREONINE ELECTROPHORESIS SEPARATION PEPTIDES CELLS HPLC Chemistry, Analytical
中文摘要	The method for simultaneous separation and determination of trace monoadenosine and diadenosine monophosphate (i.e. 2'-AMP, 3'-AMP, 5'AMP and 3'-5' ApA) in biomimicking prebiotic synthesis was developed using high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection and electrospray ionization mass spectrometry (ESI-MS) identification. The separation was performed on a Supelco C-18 column with a gradient elution (solvent A: 10mM NH4Ac aqueous solution; solvent B: MeOH). The flow rate was set at 1.0ml/min. The quantitative determination was achieved by HPLC with UV detection at 260 nm. The linearity ranged from 0.5 to 100 mu g/ml for each nucleotide. The limits of detection (LODs) for the four nucleotides were less than 0.30 mu g/ml. The recovery ranged from 95.2 to 100.7%. The intra-day relative standard deviations (RSDs) of the retention times were between 0.7 and 1.1%. Both full-scan ESI-MS and -MS2- for the four nucleotides under both positive and negative polarity were carried out and the possible cleavage pathways of them were depicted. The specific ions, [AMP+H](+) at m/z 348 and [ApA+H](+) at m/z 597, were chosen to characterize the four nucleotides in biomimicking prebiotic synthesis between N-(O,O-diisopropyl) phosphoryl amino acid (Dipp-aa) and adenosine. Using the proposed HPLC/UV/ESI-MS method, the concentration of 2'-AMP, 3'-AMP, 5'-AMP and 3'-5' ApA in the biomimicking prebiotic synthesis samples were determined. (c) 2006 Elsevier B.V. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCIENCE BV ; AMSTERDAM ; PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26345]
专题	清华大学
推荐引用方式 GB/T 7714	Liu, H,Zhao, C,Lu, JS,et al. Simultaneous measurement of trace monoadenosine and diadenosine monophosphate in biomimicking prebiotic synthesis using high-performance liquid chromatography with ultraviolet detection and electrospray ionization mass spectrometry characterization[J],2010, 2010.
APA	Liu, H.,Zhao, C.,Lu, JS.,Liu, M.,Zhang, SS.,...&Zhao, YF.(2010).Simultaneous measurement of trace monoadenosine and diadenosine monophosphate in biomimicking prebiotic synthesis using high-performance liquid chromatography with ultraviolet detection and electrospray ionization mass spectrometry characterization..
MLA	Liu, H,et al."Simultaneous measurement of trace monoadenosine and diadenosine monophosphate in biomimicking prebiotic synthesis using high-performance liquid chromatography with ultraviolet detection and electrospray ionization mass spectrometry characterization".(2010).

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