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	Aerodynamic gene gun for DNA immunization
	Yu Xinglong ; Wang Youbing ; Liu Junfeng ; Zhang Xiwen ; Wang Yuan ; He Hongxuan ; Duan Mingxing
	2010-05-10 ; 2010-05-10
关键词	Theoretical or Mathematical Experimental/ aerodynamics biomedical equipment cellular biophysics DNA genetics microfluidics molecular biophysics patient treatment shock waves stainless steel/ aerodynamic gene gun DNA immunization gene gun particle acceleration system shock waves microprojectile preparation DNA vaccines stainless steel net acceleration channel pEGFG plasmids Hela cells 300 m/s 0.3 MPa/ A8770G Patient care and treatment A8715 Molecular biophysics A8725 Cellular biophysics A4740N Shock-wave interactions E3654 Medical equipment and supplies industry E2130 Fluid mechanics and aerodynamics (mechanical engineering)/ velocity 3.0E+02 m/s pressure 3.0E+05 Pa
中文摘要	A gene gun particle acceleration system was developed to resolve problems caused by shock waves and the microprojectile preparation in previous gene guns used for DNA immunizations. In the system, the particles coated with DNA vaccines are absorbed by a stainless steel net mounted in the acceleration channel. An experimental apparatus was designed based on the simulation results to inject pEGFG plasmids into Hela cells. The experimental results show that the particles are accelerated up to 300 m/s at an air pressure of 0.3 MPa with excellent distribution uniformity on the target plane and that the pEGFG plasmids injected into the Hela cells are well expressed, which shows that the gene gun can provide effective DNA immunization.
语种	中文 ; 中文
出版者	Tsinghua Univ. Press ; China
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/24544]
专题	清华大学
推荐引用方式 GB/T 7714	Yu Xinglong,Wang Youbing,Liu Junfeng,et al. Aerodynamic gene gun for DNA immunization[J],2010, 2010.
APA	Yu Xinglong.,Wang Youbing.,Liu Junfeng.,Zhang Xiwen.,Wang Yuan.,...&Duan Mingxing.(2010).Aerodynamic gene gun for DNA immunization..
MLA	Yu Xinglong,et al."Aerodynamic gene gun for DNA immunization".(2010).

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