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	Development of an artificial neuronal network with post-mitotic rat fetal hippocampal cells by polyethylenimine
	Liu, Bingfang ; Ma, Jun ; Gao, Erjing ; He, Yu ; Cui, Fuzhai ; Xu, Qunyuan
	2010-05-10 ; 2010-05-10
关键词	hippocampus laminin micro-contact printing polyethylenimine neuronal network SOFT LITHOGRAPHY POLYELECTROLYTE MULTILAYERS PYRAMIDAL CELLS SILICON-WAFERS SURFACES CULTURE ADHESION POLYMER GROWTH PATTERNS Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology
中文摘要	The selection of appropriate surface materials that promote cellular adhesion and growth is an important consideration when designing a simplified neuronal network in vitro. In the past, extracellular matrix proteins such as laminin (LN) or positively charged substances such as POIY-L-lysine (PLL) have been used. In this study, we examined the ability of another positively charged polymer, polyethyleneimine (PEI), to promote neuronal adhesion, growth and the formation of a functional neuronal network in vitro. PEI, PLL and LN were used to produce grid-shape patterns on glass coverslips by micro-contact printing. Post-mitotic neurons from the rat fetal hippocampus were cultured on the different polymers and the viability and morphology of these neurons under serum-free culture conditions were observed using fluorescent microscopy and atomic force microscopy (AFM). We show that neurons cultured on the PEI- and PLL-coated surfaces adhered to and extended neurites along the grid-shape patterns, whereas neurons cultured on the LN-coated coverslips clustered into clumps of cells. In addition, we found that the neurons on the PEI and PLL-coated grids survived for more than 2 weeks in serum-free conditions, whereas most neurons cultured on the LN-coated grids died after 1 week. Using AFM, we observed some neurosynapse-like structures near the neuronal soma on PEI-coated coverstips. These findings indicate that PEI is a suitable surface for establishing a functional neuronal network in vitro. (c) 2007 Published by Elsevier B.V.
语种	英语 ; 英语
出版者	ELSEVIER ADVANCED TECHNOLOGY ; OXFORD ; OXFORD FULFILLMENT CENTRE THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/21054]
专题	清华大学
推荐引用方式 GB/T 7714	Liu, Bingfang,Ma, Jun,Gao, Erjing,et al. Development of an artificial neuronal network with post-mitotic rat fetal hippocampal cells by polyethylenimine[J],2010, 2010.
APA	Liu, Bingfang,Ma, Jun,Gao, Erjing,He, Yu,Cui, Fuzhai,&Xu, Qunyuan.(2010).Development of an artificial neuronal network with post-mitotic rat fetal hippocampal cells by polyethylenimine..
MLA	Liu, Bingfang,et al."Development of an artificial neuronal network with post-mitotic rat fetal hippocampal cells by polyethylenimine".(2010).

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