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	Expression of the recombinant fusion protein CP15-23 of Cryptosporidium parvum and its protective test
	He, HX ; Chen, L ; Wang, CM ; Zhou, K ; Tang, Y ; Qin, XM ; Duan, MX
	2010-05-10 ; 2010-05-10
会议名称	JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY ; 2nd International Forum on Post-Genome Technologies - Genomic Analysis and Bio-Nanoscience (2 IFPT) ; Nanjing, PEOPLES R CHINA ; Web of Science
关键词	Cryptosporidium parvum (C. parvum) CP15 CP23 fusion protein protective test Chemistry, Multidisciplinary Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Physics, Condensed Matter
中文摘要	The CP15 and CP23 surface proteins on the sporozoite of Cryptosporidium parvum are major protective antigens. The recombinant plasmid pET28-15-23 was constructed based on the plasmids pMD18-T-15 and pMD18-T-23 with two pairs of specific primers using DNA recombinant technique. In the primers, a synthetic linker sequence encoding a peptide (G-G-S) was designed. After identification, the recombinant plasmids were transformed to component cells of Escherichia coli BL21 (DE3). The positive strain containing the recombinant plasmid could express a specific fusion protein (CP15-23, MW approximate to 25 kDa) induced by IPTG. The fusion protein could be recognized by the positive serum of mice infected with Cryptosporidium parvum oocysts specifically. The BALB/c mice were immunized with 80 mu g of CP15-23 protein 4 times at 2 week intervals. The mice produced specific antibodies that responded to the lysate of Cryptosporidium parvum oocysts and could prevent Cryptosporidium parvum infection. The results indicated that the recombinant fusion protein CP15-23 would be used as a candidate antigen to prevent cryptosporidiosis.
会议录出版者	AMER SCIENTIFIC PUBLISHERS ; STEVENSON RANCH ; 25650 NORTH LEWIS WAY, STEVENSON RANCH, CA 91381-1439 USA
语种	英语 ; 英语
内容类型	会议论文
源URL	[http://hdl.handle.net/123456789/20013]
专题	清华大学
推荐引用方式 GB/T 7714	He, HX,Chen, L,Wang, CM,et al. Expression of the recombinant fusion protein CP15-23 of Cryptosporidium parvum and its protective test[C]. 见:JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2nd International Forum on Post-Genome Technologies - Genomic Analysis and Bio-Nanoscience (2 IFPT), Nanjing, PEOPLES R CHINA, Web of Science.

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