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	Photocatalytic effect on plasmid DNA damage under different UV irradiation time
	Yang, Xudong ; Wang, Ying
	2010-05-10 ; 2010-05-10
会议名称	BUILDING AND ENVIRONMENT ; 10th International Conference on Indoor Air Quality and Climate (Indoor Air 2005) ; Beijing, PEOPLES R CHINA ; Web of Science
关键词	photocatalytic oxidation (PCO) DNA damage Strand-breaking index (SBI) TITANIUM-DIOXIDE ESCHERICHIA-COLI DISINFECTION OXIDATION INACTIVATION WATER TIO2 Construction & Building Technology Engineering, Environmental Engineering, Civil
中文摘要	Microbiological contaminants such as mold and mould (both of them fungi), pathogenic bacteria, viruses that widely exist in indoor environment can cause respiratory allergic reactions, asthma, and infectious diseases ranging from influenza to Legionnaires disease. Photocatalytic oxidation (PCO) by TiO2 is a technology that can decompose a wide range of chemical pollutants and disinfect microbiological contaminants by utilizing a semiconductor catalyst such as TiO2 and near-UV (ultraviolet) radiation. In this research, a solution of supercoiled plasmid DNA was used to demonstrate photodynamic DNA strand-breaking and hydroxylation activity at photocatalyst surfaces. Index of DNA damage degree induced by a commercial TiO2 and a non-commercial nanocatalyst were compared, with the UV irradiation time as the controlled variable. Results show that as irradiation time increased, the photocatalytic effect on DNA also increased, and the nanocatalyst has a better photocatalytic effect on plasmid DNA than P25 under the same test conditions. For the irradiation time as short as five seconds, plasmid DNA damage occurred. This fundamental research provides quantitative information for further evaluation of PCO in destructing microbiological pollutants. (C) 2006 Elsevier Ltd. All rights reserved.
会议录出版者	PERGAMON-ELSEVIER SCIENCE LTD ; OXFORD ; THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND
语种	英语 ; 英语
内容类型	会议论文
源URL	[http://hdl.handle.net/123456789/19692]
专题	清华大学
推荐引用方式 GB/T 7714	Yang, Xudong,Wang, Ying. Photocatalytic effect on plasmid DNA damage under different UV irradiation time[C]. 见:BUILDING AND ENVIRONMENT, 10th International Conference on Indoor Air Quality and Climate (Indoor Air 2005), Beijing, PEOPLES R CHINA, Web of Science.

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