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	The immobilization of trypsin on soap-free P(MMA-EA-AA) latex particles
	Kang, K ; Kan, CY ; Yeung, A ; Liu, DS
	2010-05-07 ; 2010-05-07 ; OCT 04-06, 2004
会议名称	MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS ; 2nd Asia Symnposium on Biomineralization ; Beijing, PEOPLES R CHINA ; Web of Science
关键词	soap-free latex particles trypsin enzyme immobilization covalent coupling methyl methacrylate based copolymer HYDROLYSIS KINETICS ACID CELLULOSE BACILLUS CHITIN Materials Science, Multidisciplinary
中文摘要	Poly(methyl methacrylate-ethyl acrylate-acrylic acid) latex particles with narrow size distribution and with surface carboxyl groups were produced by soap-free emulsion polymerization, and covalent immobilization of trypsin onto these particles was carried out by using the water-soluble carbodiimide (EDC) as an activating agent under various conditions. Different immobilization methods were employed and the factors affecting the efficiency and activity of the immobilized enzyme, such as the amount of trypsin and EDC, pH and temperature of the immobilization reaction were investigated. Results showed that both relatively high immobilization efficiency and high enzyme activity were achieved when pre-adsorption method was employed. The immobilization efficiency decreased as the trypsin amount increased, and increased as pH and temperature increased. When the EDC amount varied, the immobilization efficiency first increased significantly and then decreased slowly. A maximum of enzyme activity can be obtained at the optimum value of 958.0 mg trypsin/g dried particles and 372.5 mg EDC/g dried particles at 25 degrees C and pH 5.0. The immobilized trypsin exhibited much higher relative activity than its free counterpart. (c) 2005 Elsevier B.V All rights reserved.; Tsinghau Univ
会议录出版者	ELSEVIER SCIENCE BV ; AMSTERDAM ; PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
语种	英语 ; 英语
内容类型	会议论文
源URL	[http://hdl.handle.net/123456789/17135]
专题	清华大学
推荐引用方式 GB/T 7714	Kang, K,Kan, CY,Yeung, A,et al. The immobilization of trypsin on soap-free P(MMA-EA-AA) latex particles[C]. 见:MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS, 2nd Asia Symnposium on Biomineralization, Beijing, PEOPLES R CHINA, Web of Science.

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