| Determination of naproxen with flow injection chemiluminescence of Ru(bpy)(3)(2+)-PbO2 system and its application for the binding study of naproxen to protein | |
| Wei, SL ; Zhao, LX ; Cheng, XL ; Lin, JM | |
| 2010-05-06 ; 2010-05-06 | |
| 关键词 | chemiluminescence flow injection naproxen drug-protein binding ultratiltration PERFORMANCE LIQUID-CHROMATOGRAPHY HUMAN-SERUM BETA-CYCLODEXTRIN FRONTAL ANALYSIS ULTRAFILTRATION DRUG MICRODIALYSIS EQUILIBRIUM TRIS(2,2'-BIPYRIDYL)RUTHENIUM(III) LUMINESCENCE Chemistry, Analytical |
| 中文摘要 | A flow injection method based on Ru(bpy)(3)(2+), -PbO2 chemiluminescence system was developed for the determination of naproxen. The factors affecting the CL intensity were performed including the amount of PbO2 and H2SO4, pH of buffer and concentration of Ru(bpy)(3)(2+). Under the optimum conditions, naproxen has a linear calibration graph in the range of 2 x 10(-8)-6 x 10(-6) mol/L with a limit of detection (S/N= 3) of 1.0 x 10(-8) mol/L, The correlation coefficient was 0.9976 (n = 12) with a relative standard deviation of 3.4% for 15 determinations of 1 X 10-6 mol/L of naproxen. Combining with ultrafiltration, this method was successfully applied to study naproxen-protein interaction. The binding constants (K) to bovine serum albumen (BSA) were K-1 = 2.06 x 106 L/mol and K-2 = 2.18 x 10(7) L/mol, and to human serum albumen (HSA) were K-1 = 4.80 x 10(6) L/mol and K-2 = 1.25 x 107 L/mol, respectively. The correlation coefficients given by Scatchard plot and Klotz plot were satisfied, indicating that studied drug has two types of binding site in the molar ratio range studied. The number of the binding site (n) on one molecule of BSA were n(1) = 6.79 and n(2) = 1.88, and of HSA were n(1) = 1.22 and n(2) = 1.80, respectively. The proposed method provided a sensitive, reliable and simple technique for studying naproxen-protein interaction and was for the first time applied to the determination of the unbound fraction of naproxen in its human serum albumin equilibrated solution. (c) 2005 Elsevier B.V. All rights reserved. |
| 语种 | 英语 ; 英语 |
| 出版者 | ELSEVIER SCIENCE BV ; AMSTERDAM ; PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/13195]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | Wei, SL,Zhao, LX,Cheng, XL,et al. Determination of naproxen with flow injection chemiluminescence of Ru(bpy)(3)(2+)-PbO2 system and its application for the binding study of naproxen to protein[J],2010, 2010. |
| APA | Wei, SL,Zhao, LX,Cheng, XL,&Lin, JM.(2010).Determination of naproxen with flow injection chemiluminescence of Ru(bpy)(3)(2+)-PbO2 system and its application for the binding study of naproxen to protein.. |
| MLA | Wei, SL,et al."Determination of naproxen with flow injection chemiluminescence of Ru(bpy)(3)(2+)-PbO2 system and its application for the binding study of naproxen to protein".(2010). |
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