Peptide sequencing through N-terminal phosphonylation and electrospray ionization mass spectrometry | |
Bao, JY ; Ai, HW ; Fu, H ; Jiang, YY ; Zhao, YF ; Huang, C | |
2010-05-06 ; 2010-05-06 | |
关键词 | peptide sequencing electrospray ionization mass spectrometry phosphonamiclate pepticles N-terminal phosphonylation ethoxyphenylphosphinate ALKALI-METAL-IONS GAS-PHASE CATIONIZED PEPTIDES CARBOXYPEPTIDASE-Y ASPARTIC-ACID PROTEINS COMPLEXES DERIVATIZATION TIME DISSOCIATION Biophysics Chemistry, Organic Spectroscopy |
中文摘要 | Peptides were phosphonylated at their N-termini by reacting with ethoxyphenylphosphinate in the presence of triethylamine and tetrachloromethane under mild conditions. The phosphonylated peptides were analyzed by tandem electrospray ionization mass spectrometry. N-Terminal phosphonylation selectively increased the intensities of b(n)-type ions relative to other ion types. The resulting simplified mass spectra clearly show the sequential loss of amino acid residues from the C-termini of peptides, providing a convenient and rapid method for peptide sequencing. Copyright (c) 2005 John Wiley & Sons, Ltd. |
语种 | 英语 ; 英语 |
出版者 | JOHN WILEY & SONS LTD ; CHICHESTER ; THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND |
内容类型 | 期刊论文 |
源URL | [http://hdl.handle.net/123456789/12525] |
专题 | 清华大学 |
推荐引用方式 GB/T 7714 | Bao, JY,Ai, HW,Fu, H,et al. Peptide sequencing through N-terminal phosphonylation and electrospray ionization mass spectrometry[J],2010, 2010. |
APA | Bao, JY,Ai, HW,Fu, H,Jiang, YY,Zhao, YF,&Huang, C.(2010).Peptide sequencing through N-terminal phosphonylation and electrospray ionization mass spectrometry.. |
MLA | Bao, JY,et al."Peptide sequencing through N-terminal phosphonylation and electrospray ionization mass spectrometry".(2010). |
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