The Effects of Cerium Oxide Incorporation in Calcium Silicate Coating on Bone Mesenchymal Stem Cell and Macrophage Responses

CORC > 上海硅酸盐研究所 > 中国科学院上海硅酸盐研究所 > 特种无机涂层重点实验室 > 期刊论文

	The Effects of Cerium Oxide Incorporation in Calcium Silicate Coating on Bone Mesenchymal Stem Cell and Macrophage Responses
	Li, Kai 1; Yu, Jiangming 2; Xie, Youtao 1; You, Mingyu 1; Huang, Liping 1; Zheng, Xuebin 1
刊名	BIOLOGICAL TRACE ELEMENT RESEARCH
	2017-05-01
卷号	177 期号:1 页码:148-158
关键词	CeO2 Calcium silicate Bone mesenchymal stem cell Macrophage
英文摘要	Ideal coatings for orthopedic implants should be able to induce excellent osseointegration with host bone tissue, which requires good osteogenic responses and limited inflammatory reactions. Cerium oxide (CeO2) ceramics have anti-oxidative properties and can be used to decrease mediators of inflammation, making them attractive for biomedical application. In this study, two kinds of CeO2 incorporated calcium silicate coatings (CS-10Ce and CS-30Ce) were prepared via plasma spraying technique, and the effects of CeO2 addition on the responses of bone mesenchymal stem cells (BMSCs) and RAW264.7 macrophages were evaluated. The CS-10Ce and CS-30Ce coatings were characterized by X-ray diffraction, scanning electron microscopy, and X-ray photoelectron spectroscopy. An increase in CeO2 content in the coatings resulted in enhanced chemical stability and better BMSCs osteogenic behaviors in terms of cell adhesion, proliferation, ALP activity, and mineralized nodule formation. With respect to either ZrO2-added or unmodified CS coating, the CS-30Ce coating elicited higher effects on the macrophages, suppressing the gene expressions of pro-inflammatory (M1) markers (CCR7, IL-6, and TNF-alpha), while upregulating the expressions of anti-inflammatory (M2) markers (CD206, IL-1ra, and IL-10); moreover, it could also increase the expression of osteoinductive molecules (BMP2 and TGF-beta 1) by the macrophages. The results suggested that the regulation of BMSCs behaviors and macrophage-mediated responses at the coating's surface was related to CeO2 incorporation. The incorporation of CeO2 in CS coatings can be a valuable strategy to promote osteogenic responses and mitigate inflammatory reactions.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Endocrinology & Metabolism
研究领域[WOS]	Biochemistry & Molecular Biology ; Endocrinology & Metabolism
关键词[WOS]	TRICALCIUM PHOSPHATE ; REGENERATIVE MEDICINE ; IN-VITRO ; DIFFERENTIATION ; NANOPARTICLES ; OSTEOBLASTS ; CERAMICS ; POLARIZATION ; BIOACTIVITY ; REPAIR
收录类别	SCI
语种	英语
WOS记录号	WOS:000398712400019
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/23791]
专题	上海硅酸盐研究所_特种无机涂层重点实验室_期刊论文
作者单位	1.Chinese Acad Sci, Key Lab Inorgan Coating Mat, Shanghai Inst Ceram, 1295 Dingxi Rd, Shanghai 200050, Peoples R China 2.Second Mil Med Univ, Dept Orthopaed, Changzheng Hosp, Shanghai 200003, Peoples R China
推荐引用方式 GB/T 7714	Li, Kai,Yu, Jiangming,Xie, Youtao,et al. The Effects of Cerium Oxide Incorporation in Calcium Silicate Coating on Bone Mesenchymal Stem Cell and Macrophage Responses[J]. BIOLOGICAL TRACE ELEMENT RESEARCH,2017,177(1):148-158.
APA	Li, Kai,Yu, Jiangming,Xie, Youtao,You, Mingyu,Huang, Liping,&Zheng, Xuebin.(2017).The Effects of Cerium Oxide Incorporation in Calcium Silicate Coating on Bone Mesenchymal Stem Cell and Macrophage Responses.BIOLOGICAL TRACE ELEMENT RESEARCH,177(1),148-158.
MLA	Li, Kai,et al."The Effects of Cerium Oxide Incorporation in Calcium Silicate Coating on Bone Mesenchymal Stem Cell and Macrophage Responses".BIOLOGICAL TRACE ELEMENT RESEARCH 177.1(2017):148-158.