High-yield hydrogen production from biomass by in vitro metabolic engineering: Mixed sugars coutilization and kinetic modeling

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	High-yield hydrogen production from biomass by in vitro metabolic engineering: Mixed sugars coutilization and kinetic modeling
	Rollin, Joseph A.1,2; del Campo, Julia Martin 1; Myung, Suwan 1,3; Sun, Fangfang 2; You, Chun 1,2; Bakovic, Allison 4; Castro, Roberto 5; Chandrayan, Sanjeev K.6; Wu, Chang-Hao 6; Adams, Michael W. W.6,7
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2015-04-21
卷号	112 期号:16 页码:4964-4969
关键词	hydrogen biomass in vitro metabolic engineering metabolic network modeling global sensitivity analysis
英文摘要	The use of hydrogen (H-2) as a fuel offers enhanced energy conversion efficiency and tremendous potential to decrease greenhouse gas emissions, but producing it in a distributed, carbon-neutral, low-cost manner requires new technologies. Herein we demonstrate the complete conversion of glucose and xylose from plant biomass to H-2 and CO2 based on an in vitro synthetic enzymatic pathway. Glucose and xylose were simultaneously converted to H-2 with a yield of two H-2 per carbon, the maximum possible yield. Parameters of a nonlinear kinetic model were fitted with experimental data using a genetic algorithm, and a global sensitivity analysis was used to identify the enzymes that have the greatest impact on reaction rate and yield. After optimizing enzyme loadings using this model, volumetric H-2 productivity was increased 3-fold to 32 mmol H-2.L-1.h(-1). The productivity was further enhanced to 54 mmol H-2.L-1.h(-1) by increasing reaction temperature, substrate, and enzyme concentrations-an increase of 67-fold compared with the initial studies using this method. The production of hydrogen from locally produced biomass is a promising means to achieve global green energy production.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	LIGNOCELLULOSE FRACTIONATION ; SYNTHETIC BIOLOGY ; CELL ; WATER ; CHEMISTRY ; ACCESSIBILITY ; CHEMICALS ; PATHWAY ; COMPLEX ; AMMONIA
收录类别	SCI
语种	英语
WOS记录号	WOS:000353239100047
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/2882]
专题	天津工业生物技术研究所_体外合成生物学研究组张以恒_期刊论文
作者单位	1.Virginia Tech, Dept Biol Syst Engn, Blacksburg, VA 24061 USA 2.Cell Free Bioinnovat, Blacksburg, VA 24060 USA 3.Virginia Tech, Inst Crit Technol & Appl Sci, Blacksburg, VA 24061 USA 4.Milwaukee Sch Engn, Dept Chem & Phys, Milwaukee, WI 53202 USA 5.Texas A&M Univ, Dept Chem & Nat Gas Engn, Kingsville, TX 78363 USA 6.Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA 7.BioEnergy Sci Ctr, Dept Energy, Oak Ridge, TN 37831 USA 8.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Rollin, Joseph A.,del Campo, Julia Martin,Myung, Suwan,et al. High-yield hydrogen production from biomass by in vitro metabolic engineering: Mixed sugars coutilization and kinetic modeling[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2015,112(16):4964-4969.
APA	Rollin, Joseph A..,del Campo, Julia Martin.,Myung, Suwan.,Sun, Fangfang.,You, Chun.,...&Zhang, Y. -H. Percival.(2015).High-yield hydrogen production from biomass by in vitro metabolic engineering: Mixed sugars coutilization and kinetic modeling.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,112(16),4964-4969.
MLA	Rollin, Joseph A.,et al."High-yield hydrogen production from biomass by in vitro metabolic engineering: Mixed sugars coutilization and kinetic modeling".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 112.16(2015):4964-4969.