Functional and structural analyses of a 1,4-beta-endoglucanase from Ganoderma lucidum

CORC > 天津工业生物技术研究所 > 结构生物学与蛋白酶学实验室郭瑞庭 > 期刊论文

	Functional and structural analyses of a 1,4-beta-endoglucanase from Ganoderma lucidum
	Liu, Guizhi 1; Li, Qian 2; Shang, Na 2; Huang, Jian-Wen 3,4; Ko, Tzu-Ping 5; Liu, Weidong 2; Zheng, Yingying 2; Han, Xu 2; Chen, Yun 6; Chen, Chun-Chi 2
刊名	ENZYME AND MICROBIAL TECHNOLOGY
	2016-05-01
卷号	86 页码:67-74
关键词	Ganoderma lucidum Endoglucanase Crystal structure
英文摘要	Ganoderma lucidum is a saprotrophic white-rot fungus which contains a rich set of cellulolytic enzymes. Here, we screened an array of potential 1,4-beta-endoglucanases from G. lucidum based on the gene annotation library and found that one candidate gene, GlCel5A, exhibits CMC-hydrolyzing activity. The recombinant GlCel5A protein expressed in Pichia pastoris is able to hydrolyze CMC and beta-glucan but not xylan and mannan. The enzyme exhibits optimal activity at 60 degrees C and pH 3-4, and retained 50% activity at 80 and 90 degrees C for at least 15 and 10 min. The crystal structure of GlCel5A and its complex with cellobiose, solved at 2.7 and 2.86 angstrom resolution, shows a classical (beta/alpha)(8) TIM-barrel fold as seen in other members of glycoside hydrolase family 5. The complex structure contains a cellobiose molecule in the +1 and +2 subsites, and reveals the interactions with the positive sites of the enzyme. Collectively, the present work provides the first comprehensive characterization of an endoglucanase from G. lucidum that possesses properties for industrial applications, and strongly encourages further studying in the cellulolytic enzyme system of G. lucidum. (C) 2016 Published by Elsevier Inc.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	THERMOASCUS-AURANTIACUS ; ENDOGLUCANASE ; SOFTWARE ; SEQUENCE ; CEL5A
收录类别	SCI
语种	英语
WOS记录号	WOS:000373650000010
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/2239]
专题	天津工业生物技术研究所_结构生物学与蛋白酶学实验室郭瑞庭_期刊论文
作者单位	1.Jiangnan Univ, Sch Biotechnol, Wuxi 214122, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Ind Enzymes Natl Engn Lab, Tianjin 300308, Peoples R China 3.Genozyme Biotechnol Inc, Taipei 196, Taiwan 4.AsiaPac Biotechnol Co Ltd, Dongguan 523808, Peoples R China 5.Acad Sinica, Inst Biol Chem, Taipei 11529, Taiwan 6.Jiangnan Univ, Sch Pharmaceut Sci, Wuxi 214122, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Liu, Guizhi,Li, Qian,Shang, Na,et al. Functional and structural analyses of a 1,4-beta-endoglucanase from Ganoderma lucidum[J]. ENZYME AND MICROBIAL TECHNOLOGY,2016,86:67-74.
APA	Liu, Guizhi.,Li, Qian.,Shang, Na.,Huang, Jian-Wen.,Ko, Tzu-Ping.,...&Guo, Rey-Ting.(2016).Functional and structural analyses of a 1,4-beta-endoglucanase from Ganoderma lucidum.ENZYME AND MICROBIAL TECHNOLOGY,86,67-74.
MLA	Liu, Guizhi,et al."Functional and structural analyses of a 1,4-beta-endoglucanase from Ganoderma lucidum".ENZYME AND MICROBIAL TECHNOLOGY 86(2016):67-74.