Validation of a HILIC Method for the Analysis of Ergothioneine in Fermentation Broth

	Validation of a HILIC Method for the Analysis of Ergothioneine in Fermentation Broth
	Liu, Qi 1; Zhang, Weiya 1,2; Wang, Hongyu 3; Li, Yunhua 3; Liu, Wei 3; Wang, Qunjie 3; Liu, Dongze 1; Chen, Ning 2; Jiang, Wenxia 1
刊名	JOURNAL OF CHROMATOGRAPHIC SCIENCE
	2016-07-01
卷号	54 期号:6 页码:934-938
英文摘要	A hydrophilic interaction liquid chromatography method has been established for the quantification of ergothioneine (EGT) in fermentation broth. Chromatographic separation was conducted on a Venusil hydrophilic interaction liquid chromatography (HILIC) column (250 x 4.6 mm, 5 mu m) at an elution rate of 1.0mL/min with an isocratic mobile phase consisting of acetonitrile/20 mmol/L ammonium acetate solution (85 : 15, v/v) adjusted to pH 6.0 with acetic acid. Analytes were detected at 254 nm using a UV-VIS detector. The injection volume was 10 mu L, and the column temperature was 40 degrees C. The limits of detection and limits of quantification were 63 and 21 mu g/L, respectively. Excellent linearity [correlation coefficient (R-2) = 0.9999] was achieved for EGT quantification in the range of 5-400 mg/L. The relative standard deviations of repeatability, intermediate precision and stability were 1.47, 1.03 and 1.66%, respectively, and EGT recoveries were within 99.2-100.8%. The chromatographic peak corresponding to EGT in the HILIC spectrum was confirmed using ESI-MS. In general, the method developed here is simple, reliable, accurate, and stable and may be useful for routine analyses in EGT biosynthesis research.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]	OXIDATIVE DAMAGE ; LIQUID-CHROMATOGRAPHY ; TNF-ALPHA ; RATS ; CELLS ; QUANTIFICATION ; SYSTEM ; BLOOD
收录类别	SCI
语种	英语
WOS记录号	WOS:000385022100007
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/2945]
专题	天津工业生物技术研究所_发酵过程姜文侠_期刊论文
作者单位	1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Key Lab Ind Biol Syst & Bioproc Engn, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 2.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300222, Peoples R China 3.Bonna Agela Technol Inc, Tianjin 300457, Peoples R China
推荐引用方式 GB/T 7714	Liu, Qi,Zhang, Weiya,Wang, Hongyu,et al. Validation of a HILIC Method for the Analysis of Ergothioneine in Fermentation Broth[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2016,54(6):934-938.
APA	Liu, Qi.,Zhang, Weiya.,Wang, Hongyu.,Li, Yunhua.,Liu, Wei.,...&Jiang, Wenxia.(2016).Validation of a HILIC Method for the Analysis of Ergothioneine in Fermentation Broth.JOURNAL OF CHROMATOGRAPHIC SCIENCE,54(6),934-938.
MLA	Liu, Qi,et al."Validation of a HILIC Method for the Analysis of Ergothioneine in Fermentation Broth".JOURNAL OF CHROMATOGRAPHIC SCIENCE 54.6(2016):934-938.