Multifunctions of dual Zn/Mg ion co-implanted titanium on osteogenesis, angiogenesis and bacteria inhibition for dental implants

CORC > 上海硅酸盐研究所 > 中国科学院上海硅酸盐研究所 > 生物材料与组织工程研究中心 > 期刊论文

	Multifunctions of dual Zn/Mg ion co-implanted titanium on osteogenesis, angiogenesis and bacteria inhibition for dental implants
	Yu, Yiqiang 1; Jin, Guodong 2; Xue, Yang 1; Wang, Donghui 2; Liu, Xuanyong 2; Sun, Jiao 1
刊名	ACTA BIOMATERIALIA
	2017-02-01
卷号	49 页码:590-603
关键词	Zinc Magnesium Ion implantation Multifunctions Dental implant
英文摘要	In order to improve the osseointegration and long-term survival of dental implants, it is urgent to develop a multifunctional titanium surface which would simultaneously have osteogeneic, angiogeneic and antibacterial properties. In this study, a potential dental implant material-dual Zn/Mg ion co-implanted titanium (Zn/Mg-PIII) was developed via plasma immersion ion implantation (PIII). The Zn/Mg-PIII surfaces were found to promote initial adhesion and spreading of rat bone marrow mesenchymal stem cells (rBMSCs) via the upregulation of the gene expression of integrin alpha 1 and integrin beta 1. More importantly, it was revealed that Zn/Mg-PIII could increase Zn2+ and Mg2+ concentrations in rBMSCs by promoting the influx of Zn2+ and Mg2+ and inhibiting the outflow of Zn2+, and then could enhance the transcription of Runx2 and the expression of ALP and OCN. Meanwhile, Mg2+ ions from Zn/Mg-PIII increased Mg2+ influx by upregulating the expression of MagT1 transporter in human umbilical vein endothelial cells (HUVECs), and then stimulated the transcription of VEGF and KDR via activation of hypoxia inducing factor (HIF)-1 alpha, thus inducing angiogenesis. In addition to this, it was discovered that zinc in Zn/Mg-PIII had certain inhibitory effects on oral anaerobic bacteria (Pg, Fn and Sm). Finally, the Zn/Mg-PIII implants were implanted in rabbit femurs for 4 and 12 weeks with Zn-PIII, Mg-PIII and pure titanium as controls. Micro-CT evaluation, sequential fluorescent labeling, histological analysis and push-out test consistently demonstrated that Zn/Mg-PIII implants exhibit superior capacities for enhancing bone formation, angiogenesis and osseointegration, while consequently increasing the bonding strength at bone-implant interfaces. All these results suggest that due to the multiple functions co-produced by zinc and magnesium, rapid osseointegration and sustained biomechanical stability are enhanced by the novel Zn/Mg-PIII implants, which have the potential application in dental implantation in the future.
WOS标题词	Science & Technology ; Technology
类目[WOS]	Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]	Engineering ; Materials Science
关键词[WOS]	MESENCHYMAL STEM-CELLS ; ALKALINE-PHOSPHATASE ACTIVITY ; FREE STAINLESS-STEEL ; ORTHOPEDIC IMPLANTS ; ZINC TRANSPORTER ; MAGNESIUM ALLOY ; BONE-FORMATION ; IN-VITRO ; SURFACE ; OSSEOINTEGRATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000394062100048
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/23726]
专题	上海硅酸盐研究所_生物材料与组织工程研究中心_期刊论文
作者单位	1.Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9, Shanghai Biomat Res & Testing Ctr,Shanghai Key La, Shanghai 200023, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China
推荐引用方式 GB/T 7714	Yu, Yiqiang,Jin, Guodong,Xue, Yang,et al. Multifunctions of dual Zn/Mg ion co-implanted titanium on osteogenesis, angiogenesis and bacteria inhibition for dental implants[J]. ACTA BIOMATERIALIA,2017,49:590-603.
APA	Yu, Yiqiang,Jin, Guodong,Xue, Yang,Wang, Donghui,Liu, Xuanyong,&Sun, Jiao.(2017).Multifunctions of dual Zn/Mg ion co-implanted titanium on osteogenesis, angiogenesis and bacteria inhibition for dental implants.ACTA BIOMATERIALIA,49,590-603.
MLA	Yu, Yiqiang,et al."Multifunctions of dual Zn/Mg ion co-implanted titanium on osteogenesis, angiogenesis and bacteria inhibition for dental implants".ACTA BIOMATERIALIA 49(2017):590-603.