Comparative study of the transfection efficiency of commonly used viral vectors in rhesus monkey (Macaca mulatta) brains

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室

	Comparative study of the transfection efficiency of commonly used viral vectors in rhesus monkey (Macaca mulatta) brains
	Wu SH 1,2; Liao ZX 1,2; Tang H 1,2; He XB 6; Wu Y 6; He XP 1,2; Yang MF 4; Li ZH 1,2; Rizak JD 1; Qin DD[*]1
刊名	Zoological Research
	2017
卷号	38 期号:2 页码:88-95
关键词	Recombinant adeno-associated virus Lentivirus Rhesus monkey Central nervous system
通讯作者	qindong108@163.com ; xthu@mail.kiz.ac.cn
英文摘要	Viral vector transfection systems are among the simplest of biological agents with the ability to transfer genes into the central nervous system. In brain research, a series of powerful and novel gene editing technologies are based on these systems. Although many viral vectors are used in rodents, their full application has been limited in non-human primates. To identify viral vectors that can stably and effectively express exogenous genes within non-human primates, eleven commonly used recombinant adeno-associated viral and lentiviral vectors, each carrying a gene to express green or red fluorescence, were injected into the parietal cortex of four rhesus monkeys. The expression of fluorescent cells was used to quantify transfection efficiency. Histological results revealed that recombinant adeno-associated viral vectors, especially the serotype 2/9 coupled with the cytomegalovirus, human synapsin I, or Ca²⁺/calmodulin-dependent protein kinase II promoters, and lentiviral vector coupled with the human ubiquitin C promoter, induced higher expression of fluorescent cells, representing high transfection efficiency. This is the first comparison of transfection efficiencies of different viral vectors carrying different promoters and serotypes in non-human primates (NHPs). These results can be used as an aid to select optimal vectors to transfer exogenous genes into the central nervous system of non-human primates.
收录类别	其他
资助信息	This study was supported by the National Program on Key Basic Research Project (973 Programs 2015CB755605) and the National Natural Science Foundation of China (81471312)
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/11031]
专题	昆明动物研究所_动物模型与人类重大疾病机理重点实验室昆明动物研究所_神经系统编码昆明动物研究所_中国科学院昆明灵长类研究中心
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China 2.Nerve System Coding Discipline Group, Kunming College of Life Science, University of the Chinese Academy of Sciences, Kunming Yunnan 650000, China 3.Kunming Primate Research Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China 4.Department of Anatomy and Histology & Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming Yunnan 650500, China 5.CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China 6.Center for Excellence in Brain Science, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan Hubei 430071, China
推荐引用方式 GB/T 7714	Wu SH,Liao ZX,Tang H,et al. Comparative study of the transfection efficiency of commonly used viral vectors in rhesus monkey (Macaca mulatta) brains[J]. Zoological Research,2017,38(2):88-95.
APA	Wu SH.,Liao ZX.,Tang H.,He XB.,Wu Y.,...&Zhang LH.(2017).Comparative study of the transfection efficiency of commonly used viral vectors in rhesus monkey (Macaca mulatta) brains.Zoological Research,38(2),88-95.
MLA	Wu SH,et al."Comparative study of the transfection efficiency of commonly used viral vectors in rhesus monkey (Macaca mulatta) brains".Zoological Research 38.2(2017):88-95.