Reciprocal chromosome painting between three laboratory rodent species

CORC > 昆明动物研究所 > 昆明动物研究所 > 遗传资源与进化国家重点实验室 > 保护遗传学(细胞库)

	Reciprocal chromosome painting between three laboratory rodent species
	Romanenko SA 1; Perelman PL 1; Serdukova NA 1; Trifonov VA 1,2; Biltueva LS 1; Wang JH 3; Li TL 3; Nie WH 3; O’Brien PCM 2; Volobouev VT 4
刊名	Mammalian Genome
	2006
卷号	17 期号:12 页码:1183-1192
通讯作者	graf@bionet.nsc.ru
合作状况	其它
英文摘要	The laboratory mouse (Mus musculus, 2n = 40), the Chinese hamster (Cricetulus griseus, 2n = 22), and the golden (Syrian) hamster (Mesocricetus auratus, 2n = 44) are common laboratory animals, extensively used in biomedical research. In contrast with the mouse genome, which was sequenced and well characterized, the hamster species has been set aside. We constructed a chromosome paint set for the golden hamster, which for the first time allowed us to perform multidirectional chromosome painting between the golden hamster and the mouse and between the two species of hamster. From these data we constructed a detailed comparative chromosome map of the laboratory mouse and the two hamster species. The golden hamster painting probes revealed 25 autosomal segments in the Chinese hamster and 43 in the mouse. Using the Chinese hamster probes, 23 conserved segments were found in the golden hamster karyotype. The mouse probes revealed 42 conserved autosomal segments in the golden hamster karyotype. The two largest chromosomes of the Chinese hamster (1 and 2) are homologous to seven and five chromosomes of the golden hamster, respectively. The golden hamster karyotype can be transformed into the Chinese hamster karyotype by 15 fusions and 3 fissions. Previous reconstructions of the ancestral murid karyotype proposed diploid numbers from 2n = 52 to 2n = 54. By integrating the new multidirectional chromosome painting data presented here with previous comparative genomics data, we can propose that syntenies to mouse Chrs 6 and 16 were both present and to hypothesize a diploid number of 2n = 48 for the ancestral Murinae/Cricetinae karyotype.
收录类别	其他
资助信息	This study was funded in part by Molecular and Cell Biology (MCB), Russian Fund for Basic Research (RFBR), Biosphere Origin and Evolution (BOE), Dy- namics of Genofonds of Plants, Animals and Human (DGPAH) research grants (ASG), an INTAS Grant to ASG and MAF-S, and a Wellcome trust grant to MAF-S. RS and PLP thank Gary Stone for additional flow sorting. RS was previously at NCI-Frederick and was partially supported by a grant from MIUR (Ministero Italiano della Universita’ e della Ricerca), ‘‘Mobility of Italian and Foreign Researchers Resid- ing Abroad.’’
语种	英语
公开日期	2010-11-10
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/6386]
专题	昆明动物研究所_保护遗传学(细胞库) 昆明动物研究所_细胞与分子进化重点实验室
作者单位	1.Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia 2.Centre for Veterinary Science, Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 OES, United Kingdom 3.Key Laboratory of Cellular & Molecular Evolution, The Chinese Academy of Sciences, Kunming, Yunnan, People’s Republic of China 4.Museum National d’Histoire Naturelle, Origine, Structure et Evolution de la Biodiversite, 55, rue Buffon, 75005 Paris, France 5.Department of Animal Biology and Genetics, University of Florence, 50122 Florence, Italy 6.The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
推荐引用方式 GB/T 7714	Romanenko SA,Perelman PL,Serdukova NA,et al. Reciprocal chromosome painting between three laboratory rodent species[J]. Mammalian Genome,2006,17(12):1183-1192.
APA	Romanenko SA.,Perelman PL.,Serdukova NA.,Trifonov VA.,Biltueva LS.,...&Graphodatsky AS[*].(2006).Reciprocal chromosome painting between three laboratory rodent species.Mammalian Genome,17(12),1183-1192.
MLA	Romanenko SA,et al."Reciprocal chromosome painting between three laboratory rodent species".Mammalian Genome 17.12(2006):1183-1192.