Akermanite bioceramics promote osteogenesis, angiogenesis and suppress osteoclastogenesis for osteoporotic bone regeneration | |
Xia, Lunguo1; Yin, Zhilan2; Mao, Lixia1; Wang, Xiuhui2; Liu, Jiaqiang1; Jiang, Xinquan3; Zhang, Zhiyuan4; Lin, Kaili2,5; Chang, Jiang2; Fang, Bing1 | |
刊名 | SCIENTIFIC REPORTS |
2016-02-25 | |
卷号 | 6 |
英文摘要 | It is a big challenge for bone healing under osteoporotic pathological condition with impaired angiogenesis, osteogenesis and remodeling. In the present study, the effect of Ca, Mg, Si containing akermanite bioceramics (Ca2MgSi2O7) extract on cell proliferation, osteogenic differentiation and angiogenic factor expression of BMSCs derived from ovariectomized rats (BMSCs-OVX) as well as the expression of osteoclastogenic factors was evaluated. The results showed that akermanite could enhance cell proliferation, ALP activity, expression of Runx2, BMP-2, BSP, OPN, OCN, OPG and angiogenic factors including VEGF and ANG-1. Meanwhile, akermanite could repress expression of osteoclastogenic factors including RANKL and TNF-alpha. Moreover, akermanite could activate ERK, P38, AKT and STAT3 signaling pathways, while crosstalk among these signaling pathways was evident. More importantly, the effect of akermanite extract on RANKL-induced osteoclastogenesis was evaluated by TRAP staining and real-time PCR assay. The results showed that akermanite could suppress osteoclast formation and expression of TRAP, cathepsin K and NFATc1. The in vivo experiments revealed that akermanite bioceramics dramatically stimulated osteogenesis and angiogenesis in an OVX rat criticalsized calvarial defect model. All these results suggest that akermanite bioceramics with the effects of Mg and Si ions on osteogenesis, angiogenesis and osteoclastogenesis are promising biomaterials for osteoporotic bone regeneration. |
WOS标题词 | Science & Technology |
类目[WOS] | Multidisciplinary Sciences |
研究领域[WOS] | Science & Technology - Other Topics |
关键词[WOS] | MESENCHYMAL STEM-CELLS ; MARROW STROMAL CELLS ; NF-KAPPA-B ; IN-VITRO ; SIGNALING PATHWAY ; CALCIUM SILICATE ; POSTMENOPAUSAL OSTEOPOROSIS ; MORPHOGENETIC PROTEIN-2 ; TRICALCIUM PHOSPHATE ; OVARIECTOMIZED RATS |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000370796600002 |
内容类型 | 期刊论文 |
源URL | [http://ir.sic.ac.cn/handle/331005/23195] |
专题 | 上海硅酸盐研究所_生物材料与组织工程研究中心_期刊论文 |
作者单位 | 1.Shanghai Jiao Tong Univ, Peoples Hosp 9, Ctr Craniofacial Orthodont, Dept Oral & Craniomaxillofacial Sci,Sch Med, Shanghai 200011, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China 3.Shanghai Jiao Tong Univ, Oral Bioengn & Regenerat Med Lab, Shanghai Res Inst Stomatol, Sch Med,Shanghai Key Lab Stomatol,Peoples Hosp 9, Shanghai 200011, Peoples R China 4.Shanghai Jiao Tong Univ, Dept Oral & Maxillofacial Head & Neck Oncol, Peoples Hosp 9, Sch Med, Shanghai 200011, Peoples R China 5.Tongji Univ, Sch & Hosp Stomatol, Shanghai Engn Res Ctr Tooth Restorat & Regenerat, Shanghai 200072, Peoples R China |
推荐引用方式 GB/T 7714 | Xia, Lunguo,Yin, Zhilan,Mao, Lixia,et al. Akermanite bioceramics promote osteogenesis, angiogenesis and suppress osteoclastogenesis for osteoporotic bone regeneration[J]. SCIENTIFIC REPORTS,2016,6. |
APA | Xia, Lunguo.,Yin, Zhilan.,Mao, Lixia.,Wang, Xiuhui.,Liu, Jiaqiang.,...&Fang, Bing.(2016).Akermanite bioceramics promote osteogenesis, angiogenesis and suppress osteoclastogenesis for osteoporotic bone regeneration.SCIENTIFIC REPORTS,6. |
MLA | Xia, Lunguo,et al."Akermanite bioceramics promote osteogenesis, angiogenesis and suppress osteoclastogenesis for osteoporotic bone regeneration".SCIENTIFIC REPORTS 6(2016). |
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