Using fluoro modified RNA aptamers as affinity ligands on magnetic beads for sensitive thrombin detection through affinity capture and thrombin catalysis

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Using fluoro modified RNA aptamers as affinity ligands on magnetic beads for sensitive thrombin detection through affinity capture and thrombin catalysis
	Hao, Lihua; Zhao, Qiang
刊名	ANALYTICAL METHODS
	2016-01
卷号	8 期号:3 页码:510-516
英文摘要	Many RNA aptamers against a variety of targets have been selected, and some of the RNA aptamers can have better binding affinity than DNA aptamers. The application of RNA aptamers against thrombin in bio-analysis previously did not attract enough attention due to the high cost in synthesis and poor stability of RNA aptamers, compared with the wide use of DNA aptamers. Here we present a sensitive and specific assay for thrombin by using a nuclease-resistant fluoro (F) modified RNA aptamer (Toggle-25) as an affinity ligand on magnetic beads to explore the capability of this RNA aptamer for thrombin detection and expand its application. This RNA aptamer had high binding affinity and good stability. The RNA aptamers were immobilized on the magnetic beads to selectively capture thrombin, and then thrombin cleaved fluorogenic or chromogenic peptide substrates. The measurement of the generated product allowed the final detection of thrombin. This assay reached a detection limit of 10 fM when a fluorogenic substrate was used. Thrombin could be successfully detected in diluted human serum. This work shows that the applied RNA aptamer Toggle-25 is a good alternative to DNA aptamers of thrombin as an affinity ligand, and it can be used in developing assays for thrombin. Other assays for thrombin can be expected by using this RNA aptamer with a different detection format.
收录类别	SCI
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/35980]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Hao, Lihua,Zhao, Qiang. Using fluoro modified RNA aptamers as affinity ligands on magnetic beads for sensitive thrombin detection through affinity capture and thrombin catalysis[J]. ANALYTICAL METHODS,2016,8(3):510-516.
APA	Hao, Lihua,&Zhao, Qiang.(2016).Using fluoro modified RNA aptamers as affinity ligands on magnetic beads for sensitive thrombin detection through affinity capture and thrombin catalysis.ANALYTICAL METHODS,8(3),510-516.
MLA	Hao, Lihua,et al."Using fluoro modified RNA aptamers as affinity ligands on magnetic beads for sensitive thrombin detection through affinity capture and thrombin catalysis".ANALYTICAL METHODS 8.3(2016):510-516.