Cellular internalization and intracellular biotransformation of silver nanoparticles in Chlamydomonas reinhardtii

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Cellular internalization and intracellular biotransformation of silver nanoparticles in Chlamydomonas reinhardtii
	Wang, Songshan; Lv, Jitao; Ma, Jingyuan; Zhang, Shuzhen
刊名	NANOTOXICOLOGY
	2016
卷号	10 期号:8 页码:1129-1135
关键词	Chlamydomonas reinhardtii HAADF-STEM NanoSIMS silver nanoparticles XAS
英文摘要	It is necessary to elucidate cellular internalization and intracellular biotransformation in order to accurately assess the toxicity and fate of nanoparticles after interaction with organisms. Therefore, this work employed a combination of high resolution imaging and in situ detection spectroscopic techniques to systematically investigate the intracellular localization, morphology and chemical speciation of silver in the cells of Chlamydomonas reinhardtii, a unicellular freshwater green alga, after exposure to AgNPs coated with polyvinylpyrrolidone at a concentration of 2.0mg/L. High resolution secondary ion mass spectrometry and high-angle annular dark field scanning transmission electron microscopy together with energy dispersive spectroscopy and selected area electron diffraction collectively confirmed that after 48h of exposure, AgNPs entered the periplasmic space after cellular internalization into the algal cells. Silver was also found to coexist with sulfur inside the cytoplasm in both crystalline and amorphous forms, which were further identified as -Ag2S and silver thiolates with synchrotron X-ray absorption spectroscopy. In combination, these analyses demonstrated that silver inside algae could be attributed to the uptake and sequestration of Ag(+)ion released from AgNPs, which was further sequestrated into cellular compartments. This study provides solid evidence for particle internalization and biotransformation of AgNPs after interaction with algae.
收录类别	SCI
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/35815]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Wang, Songshan,Lv, Jitao,Ma, Jingyuan,et al. Cellular internalization and intracellular biotransformation of silver nanoparticles in Chlamydomonas reinhardtii[J]. NANOTOXICOLOGY,2016,10(8):1129-1135.
APA	Wang, Songshan,Lv, Jitao,Ma, Jingyuan,&Zhang, Shuzhen.(2016).Cellular internalization and intracellular biotransformation of silver nanoparticles in Chlamydomonas reinhardtii.NANOTOXICOLOGY,10(8),1129-1135.
MLA	Wang, Songshan,et al."Cellular internalization and intracellular biotransformation of silver nanoparticles in Chlamydomonas reinhardtii".NANOTOXICOLOGY 10.8(2016):1129-1135.