Affinity capillary electrophoresis with laser induced fluorescence detection for thrombin analysis using nuclease-resistant RNA aptamers

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Affinity capillary electrophoresis with laser induced fluorescence detection for thrombin analysis using nuclease-resistant RNA aptamers
	Hao, Lihua; Bai, Yunlong; Wang, Hailin; Zhao, Qiang
刊名	JOURNAL OF CHROMATOGRAPHY A
	2016-12-09
卷号	1476 期号:0 页码:124-129
关键词	RNA aptamer Thrombin Affinity binding Laser induced fluorescence Capillary electrophoresis 2 '-Fluoro modification
英文摘要	Aptamer affinity capillary electrophoresis coupled with laser-induced fluorescence (CE-LIF) combines the advantages of affinity aptamer, rapid CE separation, and high sensitivity detection. Here we reported an affinity CE-LIF assay for thrombin by using a fluorophore-labeled RNA aptamer containing 2'-fluoro modification in sugar rings of pyrimidine nucleotides (C and U) as affinity ligand. This RNA aptamer has high binding affinity, specificity and biostability. Thrombin at 0.2 nM was successfully detected. This RNA aptamer allowed for the detection of thrombin spiked in diluted human serum sample due to the nuclease resistance. The RNA aptamer has comparable binding affinity to a 29-mer DNA aptamer for thrombin, and the binding site of the RNA aptamer on thrombin partially overlaps with the binding site of the 29-mer DNA aptamer on thrombin. It shows the nuclease-resistant RNA aptamers are promising in assays for thrombin. (C) 2016 Elsevier B.V. All rights reserved.
收录类别	SCI
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/35798]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Hao, Lihua,Bai, Yunlong,Wang, Hailin,et al. Affinity capillary electrophoresis with laser induced fluorescence detection for thrombin analysis using nuclease-resistant RNA aptamers[J]. JOURNAL OF CHROMATOGRAPHY A,2016,1476(0):124-129.
APA	Hao, Lihua,Bai, Yunlong,Wang, Hailin,&Zhao, Qiang.(2016).Affinity capillary electrophoresis with laser induced fluorescence detection for thrombin analysis using nuclease-resistant RNA aptamers.JOURNAL OF CHROMATOGRAPHY A,1476(0),124-129.
MLA	Hao, Lihua,et al."Affinity capillary electrophoresis with laser induced fluorescence detection for thrombin analysis using nuclease-resistant RNA aptamers".JOURNAL OF CHROMATOGRAPHY A 1476.0(2016):124-129.