Effects of two different high-fidelity DNA polymerases on genetic analysis of the cyanobacterial community structure in a subtropical deep freshwater reservoir

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 海洋生物与碳汇研究中心

	Effects of two different high-fidelity DNA polymerases on genetic analysis of the cyanobacterial community structure in a subtropical deep freshwater reservoir
	Zhen, Zhuo 1,2; Liu, Jingwen 3; Rensing, Christopher 1,4; Yan, Changzhou 1; Zhang, Yongyu 1,5
刊名	ARCHIVES OF MICROBIOLOGY
	2017
卷号	199 期号:1 页码:125-134
关键词	Cyanobacterial diversity Clone library Q5 DNA polymerase exTaq polymerase
英文摘要	The use of molecular methods to investigate the community structure and diversity of microalgae has largely replaced the previous morphological methods that were routinely carried out by microscopy. Different DNA polymerases can lead to bias in PCR amplification and affect the downstream community and diversity analysis. In this study, two clone libraries were constructed with two different DNA polymerases, Q5 high-fidelity DNA polymerase and exTaq polymerase, to compare the differences in their capability to accurately reflect the cyanobacterial community structure and diversity in a subtropical deep freshwater reservoir, Dongzhen reservoir. The results indicated that the two cyanobacterial clone libraries constructed by using Q5 high-fidelity DNA polymerase and exTaq DNA polymerase did not show significant differences, although a slightly higher community diversity was revealed by using Q5 high-fidelity DNA polymerase. It is noteworthy that so far Q5 high-fidelity DNA polymerase was the first time to be employed in the genetic analysis of cyanobacterial community. And it is for the first time that the cyanobacterial community structure in Dongzhen reservoir was analyzed using molecular methods. Phylogenetic analysis revealed that most of the clones belonged to Cyanophyta and chloroplast, among which Cyanobium sp. Suigetsu-CR5 made up the largest fraction of cyanobacteria in winter.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Microbiology
研究领域[WOS]	Microbiology
关键词[WOS]	CYLINDROSPERMOPSIS-RACIBORSKII ; EVOLUTIONARY ANALYSIS ; LYNGBYA-MAJUSCULA ; MICROBIAL MATS ; DIVERSITY ; BACTERIAL ; SEQUENCES ; ZONES ; BLOOM
收录类别	SCI
语种	英语
WOS记录号	WOS:000392121800016
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/9181]
专题	青岛生物能源与过程研究所_海洋生物与碳汇研究中心
作者单位	1.Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Xiamen 361021, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.JiMei Univ, Coll Food & Bioengn, Xiamen 361021, Peoples R China 4.Univ Copenhagen, Dept Plant & Environm Sci, Frederiksberg, Denmark 5.Chinese Acad Sci, Res Ctr Marine Biol & Carbon Sequestrat, Shandong Prov Key Lab Energy Genet, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Zhen, Zhuo,Liu, Jingwen,Rensing, Christopher,et al. Effects of two different high-fidelity DNA polymerases on genetic analysis of the cyanobacterial community structure in a subtropical deep freshwater reservoir[J]. ARCHIVES OF MICROBIOLOGY,2017,199(1):125-134.
APA	Zhen, Zhuo,Liu, Jingwen,Rensing, Christopher,Yan, Changzhou,&Zhang, Yongyu.(2017).Effects of two different high-fidelity DNA polymerases on genetic analysis of the cyanobacterial community structure in a subtropical deep freshwater reservoir.ARCHIVES OF MICROBIOLOGY,199(1),125-134.
MLA	Zhen, Zhuo,et al."Effects of two different high-fidelity DNA polymerases on genetic analysis of the cyanobacterial community structure in a subtropical deep freshwater reservoir".ARCHIVES OF MICROBIOLOGY 199.1(2017):125-134.