Application of RAD Sequencing for Evaluating the Genetic Diversity of Domesticated Panax notoginseng (Araliaceae)

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 中国科学院东亚植物多样性与生物地理学重点实验室

	Application of RAD Sequencing for Evaluating the Genetic Diversity of Domesticated Panax notoginseng (Araliaceae)
	Pan, Yuezhi1,2 ; Wang, Xueqin 3; Sun, Guiling4 ; Lie, Fusheng 2; Gong, Xun1
刊名	PLOS ONE
	2016-11-15
卷号	11 期号:11 页码:166419
英文摘要	Panax notoginseng, a traditional Chinese medicinal plant, has been cultivated and domesticated for approximately 400 years, mainly in Yunnan and Guangxi, two provinces in southwest China. This species was named according to cultivated rather than wild individuals, and no wild populations had been found until now. The genetic resources available on farms are important for both breeding practices and resource conservation. In the present study, the recently developed technology RADseq, which is based on next-generation sequencing, was used to analyze the genetic variation and differentiation of P. notoginseng. The nucleotide diversity and heterozygosity results indicated that P. notoginseng had low genetic diversity at both the species and population levels. Almost no genetic differentiation has been detected, and all populations were genetically similar due to strong gene flow and insufficient splitting time. Although the genetic diversity of P. notoginseng was low at both species and population levels, several traditional plantations had relatively high genetic diversity, as revealed by the He and p values and by the private allele numbers. These valuable genetic resources should be protected as soon as possible to facilitate future breeding projects. The possible geographical origin of Sanqi domestication was discussed based on the results of the genetic diversity analysis.
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	MULTILOCUS GENOTYPE DATA ; POPULATION-STRUCTURE ; NATURAL-POPULATIONS ; DE-NOVO ; MOLECULAR EVOLUTIONARY ; SNP DISCOVERY ; HISTORY ; DNA ; MARKERS ; PLANTS
收录类别	SCI
语种	英语
WOS记录号	WOS:000387794600061
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/33784]
专题	昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Div & Biogeog East Asia, Kunming, Peoples R China 2.Yunnan Agr Univ, Coll Agron & Biotechnol, Kunming, Peoples R China 3.Zhoukou Normal Univ, Coll Life Sci & Agron, Zhoukou, Peoples R China 4.Yunnan Key Lab Wild Plant Resources, Kunming, Peoples R China
推荐引用方式 GB/T 7714	Pan, Yuezhi,Wang, Xueqin,Sun, Guiling,et al. Application of RAD Sequencing for Evaluating the Genetic Diversity of Domesticated Panax notoginseng (Araliaceae)[J]. PLOS ONE,2016,11(11):166419.
APA	Pan, Yuezhi,Wang, Xueqin,Sun, Guiling,Lie, Fusheng,&Gong, Xun.(2016).Application of RAD Sequencing for Evaluating the Genetic Diversity of Domesticated Panax notoginseng (Araliaceae).PLOS ONE,11(11),166419.
MLA	Pan, Yuezhi,et al."Application of RAD Sequencing for Evaluating the Genetic Diversity of Domesticated Panax notoginseng (Araliaceae)".PLOS ONE 11.11(2016):166419.