The sequence variation and functional differentiation of CRDs in a scallop multiple CRDs containing lectin

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	The sequence variation and functional differentiation of CRDs in a scallop multiple CRDs containing lectin
	Huang, Mengmeng1 ; Wang, Lingling 2; Zhang, Huan 3; Yang, Chuanyan 2; Liu, Rui 3; Xu, Jiachao3 ; Jia, Zhihao 3; Song, Linsheng 2
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2017-02-01
卷号	67 页码:333-339
关键词	Chlamys farreri C-type lectin Carbohydrate-recognition domain Ca2+ binding site 2 Functional differentiation
英文摘要	A C-type lectin of multiple CRDs (CfLec-4) from Chlamys farreri was selected to investigate the sequence variation and functional differentiation of its CRDs. Its four CRDs with EPD/LSD, EPN/FAD, EPN/LND and EPN/YND key motifs were recombined separately. The recombinant proteins of CRD1 and CRD2 (designated as rCRD1 and rCRD2) could bind LPS and mannan, while the recombinant proteins of CRD3 and CRD4 (designated as rCRD3 and rCRD4) could bind LPS, PGN, mannan and glucan. Moreover, rCRD3 displayed broad microbe binding spectrum towards Gram-positive bacteria Staphylococcus aureus and Micrococcus luteus, Gram-negative bacteria Escherichia coli and Vibrio anguillarum, as well as fungi Pichia pastoris and Yarrowia lipolytica. These results indicated CRD3 contributed more to CfLec-4's nonself-recognition ability. Furthermore, CRD1, CRD3 and CRD4 functioned as opsonin participating in the clearance against invaders in scallops. The sequence variation in Ca2+ binding site 2 among CRDs was suspected to be associated with such functional differentiation. (C) 2016 Elsevier Ltd. All rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000390071200033
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/136258]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou 310018, Zhejiang, Peoples R China 2.Dalian Ocean Univ, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Huang, Mengmeng,Wang, Lingling,Zhang, Huan,et al. The sequence variation and functional differentiation of CRDs in a scallop multiple CRDs containing lectin[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,67:333-339.
APA	Huang, Mengmeng.,Wang, Lingling.,Zhang, Huan.,Yang, Chuanyan.,Liu, Rui.,...&Song, Linsheng.(2017).The sequence variation and functional differentiation of CRDs in a scallop multiple CRDs containing lectin.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,67,333-339.
MLA	Huang, Mengmeng,et al."The sequence variation and functional differentiation of CRDs in a scallop multiple CRDs containing lectin".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 67(2017):333-339.