CRISPR/Cas9-Mediated Genome Editing and Mutagenesis of EcChi4 in Exopalaemon carinicauda

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	CRISPR/Cas9-Mediated Genome Editing and Mutagenesis of EcChi4 in Exopalaemon carinicauda
	Gui, Tianshu1,2 ; Zhang, Jiquan 1,3; Song, Fengge1,2 ; Sun, Yuying 1,4; Xie, Shijun1,2 ; Yu, Kuijie 1; Xiang, Jianhai1,3
刊名	G3-GENES GENOMES GENETICS
	2016-11-01
卷号	6 期号:11 页码:3757-3764
关键词	microinjection Exopalaemon carinicauda CRISPR/Cas9 chitinase
英文摘要	The development of the type II clustered regularly interspaced short palindromic repeats (CRISPR) system has resulted in the revolution of genetic engineering, and this technology has been applied in the genome editing of various species. However, there are no reports about target-specific genome editing in shrimp. In this research, we developed a microinjection method for the ridgetail white prawn Exopalaemon carinicauda and successfully applied CRISPR/Cas9 technology to the genome editing of E. carinicauda. Through coinjection of mRNA of Cas9 nuclease and gRNA specialized for E. carinicauda chitinase 4 (EcChi4), shrimps with indel mutations were obtained. Further analysis showed that the mutations could be transmitted to the next generation. This is the first time that site-specific genome editing has been successfully demonstrated in a decapod, and will further contribute to the study of functional genomics in decapods.
收录类别	SCI
语种	英语
WOS记录号	WOS:000388678000032
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/136228]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 4.Huaihai Inst Technol, Coll Marine Life & Fisheries, Lianyungang 222005, Peoples R China
推荐引用方式 GB/T 7714	Gui, Tianshu,Zhang, Jiquan,Song, Fengge,et al. CRISPR/Cas9-Mediated Genome Editing and Mutagenesis of EcChi4 in Exopalaemon carinicauda[J]. G3-GENES GENOMES GENETICS,2016,6(11):3757-3764.
APA	Gui, Tianshu.,Zhang, Jiquan.,Song, Fengge.,Sun, Yuying.,Xie, Shijun.,...&Xiang, Jianhai.(2016).CRISPR/Cas9-Mediated Genome Editing and Mutagenesis of EcChi4 in Exopalaemon carinicauda.G3-GENES GENOMES GENETICS,6(11),3757-3764.
MLA	Gui, Tianshu,et al."CRISPR/Cas9-Mediated Genome Editing and Mutagenesis of EcChi4 in Exopalaemon carinicauda".G3-GENES GENOMES GENETICS 6.11(2016):3757-3764.