Effects of food restriction on growth, body composition and gene expression related in regulation of lipid metabolism and food intake in grass carp

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物多样性与资源保护研究中心 > 期刊论文

	Effects of food restriction on growth, body composition and gene expression related in regulation of lipid metabolism and food intake in grass carp
	Gong, Yulong 1,2; Chen, Weijun 1,2; Han, Dong 1,3; Zhu, Xiaoming 1; Yang, Yunxia 1; Jin, Junyan 1; Liu, Haokun 1; Xie, Shouqi 1
刊名	AQUACULTURE
	2017-02-20
卷号	469 页码:28-35
关键词	Grass carp (Ctenopharyngodon idellus) Ration Growth performance Lipid Food ingestion
ISSN号	0044-8486
英文摘要	It is well known that most fish would prefer to use body lipid stores for energy expenditure when receiving a long-term food restriction. However, the mechanism of this is still not clear. In the present study, a growth experiment was carried out to investigate the effects of food restriction on growth performance, gene expression related in regulation of lipid metabolism and food ingestion in grass carp (Ctenopharyngodon idellus). Four rations, satiation (S), 80% S, 60% S and 40% S, were adopted in this study. Each treatment was randomly assigned to triplicate net cages of 15 fish (177.3 +/- 3.3 g) per cage. The experiment lasted for 49 days at 30.0 +/- 3.0 degrees C. The experimental results showed that a significant increase in feeding rate and weight gain was found in grass carp with the increased ration level. The body lipid and energy content of the grass carp exhibited a significant decrease when receiving food restriction. The transcriptional levels of the genes involved in lipogenesis (srebp-1c, fas, ppar gamma) were down-regulated at the rations of food restriction. The relative expression of hepatic fas (fatty acid synthetase) and srebp-1c (sterol regulatory element-binding protein 1c) in the fish at satiation were significantly higher than the restricted-fed groups. Similarly, the expressions of hepatic ppar. (peroxisome proliferator-activated receptor-gamma) in the fish at the ration of satiation and 80% S were significantly higher than the group at the low ration of 40% S. However, the expression of hepatic cpt-1a (carnitine palmitoyl transferase I) involved in fatty acid beta-oxidation in fish was significantly up-regulated when receiving food restriction. Other hepatic lipolysis genes of ppar alpha (peroxisome proliferators-activated receptor alpha) and hl (hepatic lipase) didn't show any significant changes in restricted-fed fish. The transcriptional levels of hepatic leptin and hypothalamus pomc (proopiomelanocortin) were significantly down-regulated in fish fed with restricted rations. But the hypothalamus npy (neuropeptide Y) and lepr (leptin receptor) had no change. The present results indicated that a long-term food restriction could cause less accumulation of lipid and could be through a way of down-regulating lipogenesis genes and up-regulating lipolysis genes. Long-term restriction could also activate the appetite of grass carp by down-regulating some anorexigenic genes.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Marine & Freshwater Biology
研究领域[WOS]	Fisheries ; Marine & Freshwater Biology
关键词[WOS]	FATTY-ACID SYNTHASE ; RAINBOW-TROUT ; ENERGY BUDGET ; FEED-INTAKE ; COMPENSATORY GROWTH ; CTENOPHARYNGODON-IDELLA ; LEPTIN EXPRESSION ; SALMO-GAIRDNERI ; WHITE STURGEON ; PPAR-GAMMA
收录类别	SCI
语种	英语
WOS记录号	WOS:000391839400004
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/28864]
专题	水生生物研究所_水生生物多样性与资源保护研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Peoples R China
推荐引用方式 GB/T 7714	Gong, Yulong,Chen, Weijun,Han, Dong,et al. Effects of food restriction on growth, body composition and gene expression related in regulation of lipid metabolism and food intake in grass carp[J]. AQUACULTURE,2017,469:28-35.
APA	Gong, Yulong.,Chen, Weijun.,Han, Dong.,Zhu, Xiaoming.,Yang, Yunxia.,...&Xie, Shouqi.(2017).Effects of food restriction on growth, body composition and gene expression related in regulation of lipid metabolism and food intake in grass carp.AQUACULTURE,469,28-35.
MLA	Gong, Yulong,et al."Effects of food restriction on growth, body composition and gene expression related in regulation of lipid metabolism and food intake in grass carp".AQUACULTURE 469(2017):28-35.