Potentiometric Aptasensing of Vibrio alginolyticus Based on DNA Nanostructure-Modified Magnetic Beads

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Potentiometric Aptasensing of Vibrio alginolyticus Based on DNA Nanostructure-Modified Magnetic Beads
	Zhao, Guangtao 1,2,3; Ding, Jiawang1,2 ; Yu, Han 1,2,3; Yin, Tanji1,2 ; Qin, Wei1,2 ; Yin, TJ
刊名	SENSORS
	2016-12-01
卷号	16 期号:12 页码:.
关键词	potentiometric aptasensing Vibrio alginolyticus DNA nanostructures magnetic beads protamine
ISSN号	1424-8220
通讯作者	Yin, TJ ; Qin, W (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China. ; Yin, TJ ; Qin, W (reprint author), YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China. gtzhao@yic.ac.cn ; jwding@yic.ac.cn ; hyu@yic.ac.cn ; tjyin@yic.ac.cn ; wqin@yic.ac.cn
产权排序	[Zhao, Guangtao; Ding, Jiawang; Yu, Han; Yin, Tanji; Qin, Wei] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China; [Zhao, Guangtao; Ding, Jiawang; Yu, Han; Yin, Tanji; Qin, Wei] YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China; [Zhao, Guangtao; Yu, Han] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
英文摘要	A potentiometric aptasensing assay that couples the DNA nanostructure-modified magnetic beads with a solid-contact polycation-sensitive membrane electrode for the detection of Vibrio alginolyticus is herein described. The DNA nanostructure-modified magnetic beads are used for amplification of the potential response and elimination of the interfering effect from a complex sample matrix. The solid-contact polycation-sensitive membrane electrode using protamine as an indicator is employed to chronopotentiometrically detect the change in the charge or DNA concentration on the magnetic beads, which is induced by the interaction between Vibrio alginolyticus and the aptamer on the DNA nanostructures. The present potentiometric aptasensing method shows a linear range of 10-100 CFU mL(-1) with a detection limit of 10 CFU mL(-1), and a good specificity for the detection of Vibrio alginolyticus. This proposed strategy can be used for the detection of other microorganisms by changing the aptamers in the DNA nanostructures.
学科主题	Chemistry ; Electrochemistry ; Instruments & Instrumentation
研究领域[WOS]	Chemistry ; Electrochemistry ; Instruments & Instrumentation
关键词[WOS]	IONIC LIQUID ; CARBON NANOTUBES ; ELECTRODE ; APTAMERS ; ASSAYS ; RECOGNITION ; PERFORMANCE ; INFECTIONS ; BIOSENSOR ; PROTAMINE
收录类别	SCI
语种	英语
WOS记录号	WOS:000391303000072
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/19248]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Yin, TJ
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China 2.YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Guangtao,Ding, Jiawang,Yu, Han,et al. Potentiometric Aptasensing of Vibrio alginolyticus Based on DNA Nanostructure-Modified Magnetic Beads[J]. SENSORS,2016,16(12):..
APA	Zhao, Guangtao,Ding, Jiawang,Yu, Han,Yin, Tanji,Qin, Wei,&Yin, TJ.(2016).Potentiometric Aptasensing of Vibrio alginolyticus Based on DNA Nanostructure-Modified Magnetic Beads.SENSORS,16(12),..
MLA	Zhao, Guangtao,et al."Potentiometric Aptasensing of Vibrio alginolyticus Based on DNA Nanostructure-Modified Magnetic Beads".SENSORS 16.12(2016):..