Analysis of cancer marker in tissues with hadamard transform fluorescence spectral microscopic imaging (EI)

CORC > 寒区旱区环境与工程研究所 > 中科院寒区旱区环境与工程研究所（未分类） > 期刊论文

	Analysis of cancer marker in tissues with hadamard transform fluorescence spectral microscopic imaging (EI)
	Xu H.; Chen C.; He Y.; Tang H.-W.; Zhang Z.-L.; Li Y.; Pang D.-W.
刊名	Journal of Fluorescence
	2015
卷号	25 期号:2 页码:397-402
英文摘要	Quantum dots (QDs) probes were used to tag and trace cancer biomarkers in cancer tissues based on the system of home-made Hadamard transform (HT) spectral microscopic imaging, which can be applied to provide high-resolution fluorescence spectrum and image of single cells and tissues. In situ fluorescence imaging for cancer marker proteins, such as estrogen receptor (ER), human epidermal growth factor receptor 2 (HER2), proliferating cell nuclear antigen (PCNA) and cytokeratin 20 (CK20) in tumor tissues, were realized by using the HT system to capture quantitative information for these proteins when tumor tissues were immunostained with QDs probes. A method to evaluate tumor malignancy of the specimens based on in situ analysis of distribution of marker proteins was proposed based on the comparative study of positive samples and negative controls. The investigation of ER contents of the cores in breast cancer tissue microarrays (TMAs) shows that the technique of QDs-immunohistochemistry (IHC)/HT spectral imaging is more sensitive than conventional IHC method. The results also demonstrate that the QDs-IHC/HT spectral imaging technique can be applied to visualize and quantitatively measure the subcellular molecules inside tumor tissues, and the coupling of HT spectral imaging to the probing of subcellular molecules with QDs has great potential in biology and medical diagnosis. 2015 Springer Science+Business Media New York.
收录类别	EI
语种	英语
内容类型	期刊论文
源URL	[http://ir.casnw.net/handle/362004/27120]
专题	寒区旱区环境与工程研究所_中科院寒区旱区环境与工程研究所（未分类）_期刊论文
推荐引用方式 GB/T 7714	Xu H.,Chen C.,He Y.,et al. Analysis of cancer marker in tissues with hadamard transform fluorescence spectral microscopic imaging (EI)[J]. Journal of Fluorescence,2015,25(2):397-402.
APA	Xu H..,Chen C..,He Y..,Tang H.-W..,Zhang Z.-L..,...&Pang D.-W..(2015).Analysis of cancer marker in tissues with hadamard transform fluorescence spectral microscopic imaging (EI).Journal of Fluorescence,25(2),397-402.
MLA	Xu H.,et al."Analysis of cancer marker in tissues with hadamard transform fluorescence spectral microscopic imaging (EI)".Journal of Fluorescence 25.2(2015):397-402.