Surface modification of paclitaxel-loaded tri-block copolymer PLGA-b-PEG-b-PLGA nanoparticles with protamine for liver cancer therapy

	Surface modification of paclitaxel-loaded tri-block copolymer PLGA-b-PEG-b-PLGA nanoparticles with protamine for liver cancer therapy
	Nansha Gao; Zhihong Chen; Xiaojun Xiao; Changshun Ruan; Lin Mei; Zhigang Liu; Xiaowei Zeng
刊名	JOURNAL OF NANOPARTICLE RESEARCH
	2015
英文摘要	In order to enhance the therapeutic effect of chemotherapy on liver cancer, a biodegradable formulation of protamine-modified paclitaxel-loadedpoly(lactide-co-glycolide)-b-poly(ethylene glycol)-b-poly(lactide-co-glycolide) (PLGA-b-PEG-b-PLGA) nanoparticles (PTX-loaded/protamine NPs) was prepared. Tri-block copolymer PLGA-b-PEG-b-PLGA was synthesized by ring-opening polymerization and characterized by H-1 NMR spectroscopy and gel permeation chromatography. PTX-loaded and PTX-loaded/protamine NPs were characterized in terms of size, size distribution, zeta potential,surface morphology, drug encapsulation efficiency, and drug release. Confocal laser scanning microscopy showed that coumarin 6-loaded/protamineNPs were internalized by hepatocellular carcinoma cell line HepG2. The cellular uptake efficiency of NPs was obviously elevated after protaminemodification. With commercial formulation Taxol(R) as the reference, HepG2 cells were also used to study the cytotoxicity of the NPs. PTX-loaded/protamine NPs exhibited significantly higher cytotoxicity than PTX-loaded NPs and Taxol(R) did. All the results suggested that surfacemodification of PTX-loaded PLGA-b-PEG-b-PLGA NPs with protamine boosted the therapeutic efficacy on liver cancer.
收录类别	SCI
原文出处	http://download.springer.com/static/pdf/485/art%253A10.1007%252Fs11051-015-3121-3.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs11051-015-3121-3&token2=exp=1442973149~acl=%2Fstatic%2Fpdf%2F485%2Fart%25253A10.1007%25252Fs11051-015-3121-3.pdf%3ForiginUrl%3Dhttp%253A%252F%252Flink.springer.com%252Farticle%252F10.1007%252Fs11051-015-3121-3*~hmac=35023af6c0e99f81eda8b02a6e2c0eb6cd6e85f370bab4d1e8c2c9a993c15dd6
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/7386]
专题	深圳先进技术研究院_医药所
作者单位	JOURNAL OF NANOPARTICLE RESEARCH
推荐引用方式 GB/T 7714	Nansha Gao,Zhihong Chen,Xiaojun Xiao,et al. Surface modification of paclitaxel-loaded tri-block copolymer PLGA-b-PEG-b-PLGA nanoparticles with protamine for liver cancer therapy[J]. JOURNAL OF NANOPARTICLE RESEARCH,2015.
APA	Nansha Gao.,Zhihong Chen.,Xiaojun Xiao.,Changshun Ruan.,Lin Mei.,...&Xiaowei Zeng.(2015).Surface modification of paclitaxel-loaded tri-block copolymer PLGA-b-PEG-b-PLGA nanoparticles with protamine for liver cancer therapy.JOURNAL OF NANOPARTICLE RESEARCH.
MLA	Nansha Gao,et al."Surface modification of paclitaxel-loaded tri-block copolymer PLGA-b-PEG-b-PLGA nanoparticles with protamine for liver cancer therapy".JOURNAL OF NANOPARTICLE RESEARCH (2015).