Src siRNA prevents corticosteroid-associated osteoporosis in a rabbit model

	Src siRNA prevents corticosteroid-associated osteoporosis in a rabbit model
	Zheng, Li-Zhen; Wang, Xin-Luan; Cao, Hui-Juan; Chen, Shi-Hui; Huang, Le; Qin, Ling
刊名	BONE
	2016
英文摘要	In an established steroid-associated osteonecrosis (SAON) rabbit model we found recently that blockage Src by siRNA could improve reconstructive repair of osteonecrosis via enhancing osteogenesis and inhibiting bone resorption. The current study investigated if blocking Src was able to prevent steroid-associated osteoporosis (SAOP) in the same SAON animal model. Rabbits were treated with pulsed lipopolysaccharide (LPS) and corticosteroidmethylprednisolone (MPS). At 2, 4, and 6 weeks after induction, Src siRNA, control siRNA and saline were intramedullary injected into proximal femur, respectively. Two fluorescent dyes xylenol orange and calcein green were injected before sacrificing the animals for in vivo labeling of the newly formed bone. At week 6 after induction, proximal femora of rabbits were dissected for micro-CT and histological analysis. Results showed significant bone lossin the metaphysis of femoral head in the control rabbits after SAON induction. Src siRNA treatment was able to prevent steroid-associate bone loss intrabecular bone and increase cortical bone thickness at femoral neck. Histomorphometry showed that Src siRNA increased the osteoblastic bone formation and decreased the eroded bone surfaces suggesting decreased osteoclastic bone resorption. This was the first study to report bone loss after SAON induction in rabbit model that could be prevented by knocking down Src by siRNA.
收录类别	SCI
原文出处	http://www.sciencedirect.com/science/article/pii/S8756328215004111
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/10521]
专题	深圳先进技术研究院_医工所
作者单位	BONE
推荐引用方式 GB/T 7714	Zheng, Li-Zhen,Wang, Xin-Luan,Cao, Hui-Juan,et al. Src siRNA prevents corticosteroid-associated osteoporosis in a rabbit model[J]. BONE,2016.
APA	Zheng, Li-Zhen,Wang, Xin-Luan,Cao, Hui-Juan,Chen, Shi-Hui,Huang, Le,&Qin, Ling.(2016).Src siRNA prevents corticosteroid-associated osteoporosis in a rabbit model.BONE.
MLA	Zheng, Li-Zhen,et al."Src siRNA prevents corticosteroid-associated osteoporosis in a rabbit model".BONE (2016).