Biofabrication of a PLGA-TCP-based porous bioactive bone substitute with sustained release of icaritin

	Biofabrication of a PLGA-TCP-based porous bioactive bone substitute with sustained release of icaritin
	Xie XH; Wang XL; Zhang G; He YX; Leng Y; Tang TT; Pan X; Qin L
刊名	JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
	2015
英文摘要	A phytomolecule, icaritin, has been identified and shown to be osteopromotive for the prevention of osteoporosis and osteonecrosis. This study aimed to produce a bioactive poly (l-lactide-co-glycolide)-tricalcium phosphate (PLGA-TCP)-based porous scaffold incorporating the osteopromotive phytomoleculeicaritin, using a fine spinning technology. Both the structure and the composition of icaritin-releasing PLGA-TCP-based scaffolds were evaluated by scanning electron microscopy (SEM). The porosity was quantified by both water absorption and micro-computed tomography (micro-CT). The mechanical properties were evaluated using a compression test. In vitro release of icaritin from the PLGA-TCP scaffold was quantified by high-performance liquid chromatography (HPLC). The attachment, proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) on the composite scaffold were evaluated. Both an in vitro cytotoxicity test and an in vivo test via muscular implantation were conducted to confirm the scaffold's biocompatibility. The results showed that the PLGA-TCP-icaritin composite scaffold was porous, with interconnected macro- (about 480 mu m) and micropores (2-15 mu m). The mechanical properties of the PLGA-TCP-icaritin scaffold were comparable with those of the pure PLGA-TCP scaffold, yet was spinning direction-dependent. Icaritin content was detected in the medium and increased with time. The PLGA-TCP-icaritinscaffold facilitated the attachment, proliferation and osteogenic differentiation of BMSCs. In vitro cytotoxicity test and in vivo intramuscular implantation showed that the composite scaffold had no toxicity with good biocompatibility. In conclusion, an osteopromotive phytomolecule, icaritin, was successfully incorporated into PLGA-TCP to form an innovative porous composite scaffold with sustained release of osteopromotive icaritin, and this scaffold had good biocompatibility and osteopromotion, suggesting its potential for orthopaedic applications
收录类别	SCI
原文出处	http://www.ncbi.nlm.nih.gov/pubmed/23255530
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/7193]
专题	深圳先进技术研究院_医工所
作者单位	JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
推荐引用方式 GB/T 7714	Xie XH,Wang XL,Zhang G,et al. Biofabrication of a PLGA-TCP-based porous bioactive bone substitute with sustained release of icaritin[J]. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE,2015.
APA	Xie XH.,Wang XL.,Zhang G.,He YX.,Leng Y.,...&Qin L.(2015).Biofabrication of a PLGA-TCP-based porous bioactive bone substitute with sustained release of icaritin.JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE.
MLA	Xie XH,et al."Biofabrication of a PLGA-TCP-based porous bioactive bone substitute with sustained release of icaritin".JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE (2015).