Induced apoptosis of osteoblasts proliferating on polyhydroxyalkanoates

	Induced apoptosis of osteoblasts proliferating on polyhydroxyalkanoates
	Wang Yang; Jiang Xian-Li; Peng Si-Wu; Guo Xiao-Yong; Shang Guan-Guan; Chen Jin-Chun; Wu Qiong; Chen Guo-Qiang
刊名	BIOMATERIALS
	2013
英文摘要	The mechanism study on behaviors of cells influenced by biomaterial surface properties can provide profound guidances for functional tissue engineering scaffolds design. In this study, regulation of integrin-mediated cell–substrate interactions using rat osteoblasts incubated on PHA films was investigated. Compared with tissue culture plate (TCP), poly-3-hydroxybutyrate (PHB), copolymer of 3-hydroxybutyrate and 3-hydroxyvalerate (PHBV) and copolymer of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx), osteoblasts inoculated on a terpolymer of 3-hydroxybutyrate, 3-hydroxyvalerate and 3-hydroxyhexanoate (PHBVHHx) were found to have higher apoptosis rates. Several integrin subunits in osteoblasts grown on PHBVHHx showed altered expressions. Simultaneously, extracellular matrics (ECM) were also remodeled on the material surface. Osteoblasts showed a higher expression of integrin subunit β3 and αv on PHBVHHx films compared with that on TCP. On the other hand, less vitronectin, osteopontin and fibronectin, the main ligands for integrin β3 were expressed and deposited in ECM. The unligated integrin β3 could recruit caspase-8 to the membrane and activate its downstream signaling which was proven by the caspase-8 activation assay. It was therefore concluded that the induced apoptosis of osteoblasts on PHBVHHx was regulated by recruitment of caspase-8 to the unligated integrin β3.
收录类别	SCI
原文出处	http://www.sciencedirect.com/science/article/pii/S0142961213001439
语种	英语
WOS记录号	WOS:000317168700001
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/4914]
专题	深圳先进技术研究院_医工所
作者单位	BIOMATERIALS
推荐引用方式 GB/T 7714	Wang Yang,Jiang Xian-Li,Peng Si-Wu,et al. Induced apoptosis of osteoblasts proliferating on polyhydroxyalkanoates[J]. BIOMATERIALS,2013.
APA	Wang Yang.,Jiang Xian-Li.,Peng Si-Wu.,Guo Xiao-Yong.,Shang Guan-Guan.,...&Chen Guo-Qiang.(2013).Induced apoptosis of osteoblasts proliferating on polyhydroxyalkanoates.BIOMATERIALS.
MLA	Wang Yang,et al."Induced apoptosis of osteoblasts proliferating on polyhydroxyalkanoates".BIOMATERIALS (2013).