Bone marrow stromal cells with a combined expression of BMP-2 and VEGF-165 enhanced bone regeneration

	Bone marrow stromal cells with a combined expression of BMP-2 and VEGF-165 enhanced bone regeneration
	Caiwen Xiao; Huifang Zhou; Guangpeng Liu; Peng Zhang; Yao Fu; Ping Gu; Hongliang Hou; Tingting Tang and Xianqun Fan
刊名	BIOMEDICAL MATERIALS
	2011
卷号	6 期号:1
英文摘要	Bone graft substitutes with osteogenic factors alone often exhibit poor bone regeneration due to inadequate vascularization. Combined delivery of osteogenic and angiogenic factors from biodegradable scaffolds may enhance bone regeneration. We evaluated the effects of bone morphogenetic protein 2 (BMP2) and vascular endothelial growth factor (VEGF), combined with natural coral scaffolds, on the repair of critical-sized bone defects in rabbit orbits. In vitro expanded rabbit bone marrow stromal cells (BMSCs) were transfected with human BMP2 and VEGF165 genes. Target protein expression and osteogenic differentiation were confirmed after gene transduction. Rabbit orbital defects were treated with a coral scaffold loaded with BMP2-transduced and VEGF-transduced BMSCs, BMP2-expressing BMSCs, VEGF-expressing BMSCs, or BMSCs without gene transduction. Volume and density of regenerated bone were determined by micro-computed tomography at 4, 8, and 16 weeks after implantation. Neovascularity, new bone deposition rate, and new bone formation were measured by immunostaining, tetracycline and calcein labelling, and histomorphometric analysis at different time points. The results showed that VEGF increased blood vessel formation relative to groups without VEGF. Combined delivery of BMP2 and VEGF increased new bone deposition and formation, compared with any single factor. These findings indicate that mimicking the natural bone development process by combined BMP2 and VEGF delivery improves healing of critical-sized orbital defects in rabbits.
收录类别	SCI
原文出处	http://iopscience.iop.org/1748-605X/6/1/015013
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/3483]
专题	深圳先进技术研究院_医工所
作者单位	BIOMEDICAL MATERIALS
推荐引用方式 GB/T 7714	Caiwen Xiao,Huifang Zhou,Guangpeng Liu,et al. Bone marrow stromal cells with a combined expression of BMP-2 and VEGF-165 enhanced bone regeneration[J]. BIOMEDICAL MATERIALS,2011,6(1).
APA	Caiwen Xiao.,Huifang Zhou.,Guangpeng Liu.,Peng Zhang.,Yao Fu.,...&Tingting Tang and Xianqun Fan.(2011).Bone marrow stromal cells with a combined expression of BMP-2 and VEGF-165 enhanced bone regeneration.BIOMEDICAL MATERIALS,6(1).
MLA	Caiwen Xiao,et al."Bone marrow stromal cells with a combined expression of BMP-2 and VEGF-165 enhanced bone regeneration".BIOMEDICAL MATERIALS 6.1(2011).