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	Two-photon excited fluorescence emission from hemoglobin
	Sun Qiqi; Zeng Yan; Zhang Wei; Zheng Wei; Luo Yi; Qu Jianan Y.
	2015
会议名称	Conference on Multiphoton Microscopy in the Biomedical Sciences XV 2015
会议地点	9329
英文摘要	Hemoglobin, one of the most important proteins in blood, is responsible for oxygen transportation in almost all vertebrates. Recently, we discovered two-photon excited hemoglobin fluorescence and achieved label-free microvascular imaging based on the hemoglobin fluorescence. However, the mechanism of its fluorescence emission still remains unknown. In this work, we studied the two-photon excited fluorescence properties of the hemoglobin subunits, heme/hemin (iron (II)/(III) protoporphyrin IX) and globin. We first studied the properties of heme and the similar spectral and temporal characteristics of heme and hemoglobin fluorescence provide strong evidence that heme is the fluorophore in hemoglobin. Then we studied the fluorescence properties of hemin, globin and methemoglobin, and found that the hemin may have the main effect on the methemoglobin fluorescence and that globin has tryptophan fluorescence like other proteins. Finally, since heme is a centrosymmetric molecule, that the Soret band fluorescence of heme and hemoglobin was not observed in the single photon process in the previous study may be due to the parity selection rule. The discovery of heme two-photon excited fluorescence may open a new window for heme biology research, since heme as a cofactor of hemoprotein has many functions, including chemical catalysis, electron transfer and diatomic gases transportation.
收录类别	ISTP
语种	英语
内容类型	会议论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/7212]
专题	深圳先进技术研究院_医工所
作者单位	2015
推荐引用方式 GB/T 7714	Sun Qiqi,Zeng Yan,Zhang Wei,et al. Two-photon excited fluorescence emission from hemoglobin[C]. 见:Conference on Multiphoton Microscopy in the Biomedical Sciences XV 2015. 9329.

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