Direct detection of optogenetically evoked oscillatory neuronal electrical activity in rats using SLOE sequence

	Direct detection of optogenetically evoked oscillatory neuronal electrical activity in rats using SLOE sequence
	Chai, Yuhui; Bi, Guoqiang; Wang, Liping; Xu, Fuqiang; Wu, Ruiqi; Zhou, Xin; Qiu, Bensheng; Lei, Hao; Zhang, Yaoyu; Gao, Jia-Hong
刊名	NEUROIMAGE
	2016
英文摘要	The direct detection of neuronal electrical activity is one of the most challenging goals in non-BOLD fMRI research. Previous work has demonstrated its feasibility in phantom and cell culture studies, but attempts in in vivo studies remain fewand far between. Most recent in vivo studies used T2*-weighted sequences to directly detect neuronal electrical activity evoked by sensory stimulus. As neuronal electrical signal is usually comprised of a series ofspectrally distributed oscillatorywaveforms rather than being a direct current, it is most likely to be detected using oscillatory current sensitive sequences. In this study, we explored the potential of using the spin-lock oscillatory excitation (SLOE) sequence with spiral readout to directly detectoptogenetically evoked oscillatory neuronal electrical activity, whose main spectral component can be manipulated artificially to match the resonance frequency of spin-lock RF field. In addition, experiments using the stimulus-induced rotary saturation (SIRS) sequence with spiral readout were also performed. Electrophysiological recording and MRI data acquisition were conducted on separate animals. Robust optogenetically evoked oscillatory LFP signals were observed and significant BOLD signals were acquired with the GE-EPI sequence before and after the whole SLOE and SIRS acquisitions, but no significant neuronal current MRI (ncMRI) signal changes were detected. These results indicate that the sensitivity of oscillatory current sensitive sequences needs to be further improved for direct detection of neuronal electrical activity.
收录类别	SCI
原文出处	http://www.sciencedirect.com/science/article/pii/S1053811915009702
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/9782]
专题	深圳先进技术研究院_脑所
作者单位	NEUROIMAGE
推荐引用方式 GB/T 7714	Chai, Yuhui,Bi, Guoqiang,Wang, Liping,et al. Direct detection of optogenetically evoked oscillatory neuronal electrical activity in rats using SLOE sequence[J]. NEUROIMAGE,2016.
APA	Chai, Yuhui.,Bi, Guoqiang.,Wang, Liping.,Xu, Fuqiang.,Wu, Ruiqi.,...&Gao, Jia-Hong.(2016).Direct detection of optogenetically evoked oscillatory neuronal electrical activity in rats using SLOE sequence.NEUROIMAGE.
MLA	Chai, Yuhui,et al."Direct detection of optogenetically evoked oscillatory neuronal electrical activity in rats using SLOE sequence".NEUROIMAGE (2016).