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	A centrifugal microfluidics platform for potential application on immobilization-free bead-based immunoassays
	Chen, Qiu-lan; Cheung, Ka-lun; Kwan, Yiu-wa; Kong, Siu-kai; Ho, Ho-pui
	2012
会议名称	2nd International Forum on Micro Manufacturing, IFMM 2012
会议地点	S China Univ Techn, Guangzhou, PEOPLES R CHINA
英文摘要	In this work, we demonstrate a simple yet convenient centrifugal microfluidics, or "lab-on-a-disc (LOAD)" platform for bead-based immunoassays. The disc contains a network of passive valves for sample manipulation and a bead-aggregating barrier structure to instead of commonly used immobilization method. The narrow-channel structure not only provides a geometric barrier for retaining the beads, but also significantly increased fluidic resistance which results in lower flow rate and longer reaction time for each reagent in the reaction chamber. Centrifuge-forced beads densification in the small junction area and signal concentrating allows high signal sensing capability. The acceleration speed is optimized for largest signal-to-noise ratio. The device uses different rotation speed to trigger a cascade of flow events. The integrated platform contains all the necessary functional elements for conducting one-step parallel assays through a sequential protocol. For our test device, the total time required to complete an immunoassay experiment is typically under 6 minutes. Through this work we have also shown that the use of a geometric barrier for the aggregation of signaling beads is an effective approach for the enhancement of optical signals in common immunoassays. This flexible design is suitable for a broad range of bead-based immunoassays with low-cost and high-speed operation.
收录类别	EI
语种	英语
内容类型	会议论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/4346]
专题	深圳先进技术研究院_南沙所
作者单位	2012
推荐引用方式 GB/T 7714	Chen, Qiu-lan,Cheung, Ka-lun,Kwan, Yiu-wa,et al. A centrifugal microfluidics platform for potential application on immobilization-free bead-based immunoassays[C]. 见:2nd International Forum on Micro Manufacturing, IFMM 2012. S China Univ Techn, Guangzhou, PEOPLES R CHINA.

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