Interrogation of Cellular Innate Immunity by Diamond-Nanoneedle-Assisted Intracellular Molecular Fishing

	Interrogation of Cellular Innate Immunity by Diamond-Nanoneedle-Assisted Intracellular Molecular Fishing
	Zixun Wang; Yang Yang; Zhen Xu; Ying Wang; Wenjun Zhang; Peng Shi
刊名	NANO LETTERS
	2015
英文摘要	Understanding intracellular signaling cascades and network is one of the core topics in modern biology. Novel tools based on nanotechnologies have enabled probing and analyzing intracellular signaling with unprecedented sensitivity and specificity. In this study, we developed a minimally invasive method for in situ probing specific signaling components of cellular innate immunity in living cells. The technique was based on diamond-nanoneedlearrays functionalized with aptamer-based molecular sensors, which were inserted into cytoplasmic domain using a centrifugation controlled process to capture molecular targets. Simultaneously, these diamond-nanoneedles also facilitated the delivery of double-strand DNAs (dsDNA90) into cells to activate the pathway involving the stimulator of interferon genes (STING). We showed that the nanoneedle-based biosensors can be successfully utilized to isolate transcriptional factor, NF-kappa B, from intracellular regions without damaging the cells, upon STING activation. By using a reversible protocol and repeated probing in living cells, we were able to examine the singling dynamics of NF-kappa B, which was quickly translocated from cytoplasm to nucleus region within similar to 40 min of intracellular introduction of dsDNA90 for both A549 and neuron cells. These results demonstrated a novel and versatile tool for targeted in situ dissection of intracellular signaling, providing the potential to resolve new sights into various cellular processes.
收录类别	SCI
原文出处	http://pubs.acs.org/doi/abs/10.1021/acs.nanolett.5b03126
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/6485]
专题	深圳先进技术研究院_集成所
作者单位	NANO LETTERS
推荐引用方式 GB/T 7714	Zixun Wang,Yang Yang,Zhen Xu,et al. Interrogation of Cellular Innate Immunity by Diamond-Nanoneedle-Assisted Intracellular Molecular Fishing[J]. NANO LETTERS,2015.
APA	Zixun Wang,Yang Yang,Zhen Xu,Ying Wang,Wenjun Zhang,&Peng Shi.(2015).Interrogation of Cellular Innate Immunity by Diamond-Nanoneedle-Assisted Intracellular Molecular Fishing.NANO LETTERS.
MLA	Zixun Wang,et al."Interrogation of Cellular Innate Immunity by Diamond-Nanoneedle-Assisted Intracellular Molecular Fishing".NANO LETTERS (2015).