Selective extraction of endogenous peptides from human serum with magnetic mesoporous carbon composites | |
Gan, Yangyang1; Zhang, Quanqing2,3; Chen, Yajing1; Zhao, Yiman1; Xiong, Zhichao4; Zhang, Lingyi1; Zhang, Weibing1 | |
刊名 | TALANTA |
2016-12-01 | |
卷号 | 161页码:647-654 |
关键词 | Mesoporous carbon material Magnetic Endogenous peptides Human serum |
英文摘要 | Highly sensitive and selective enrichment of endogenous peptides or proteins from complex bio-system takes a significant important place to the proteomic. In this work, a unique Fe3O4@2SiO(2)@mSiO(2)-C nanomaterial was synthesized, contributing to the separation and enrichment of low concentration peptides from complex mixture. The highly ordered mesoporous carbon structure render the nanospheres with unique properties of strongly connected pore channels, strong hydrophobic properties, high specific surface area (254.90 m(2)/g), uniform pore size (3.61 nm). Which made it a promising candidates for the efficient enrichment of peptides through hydrophobic-hydrophobic interaction with low detection limit (0.2 fmol), superb size-exclusion of high molecular weight proteins, highly selectivity for BSA digest (molar ratio of BSA tryptic digests/BSA, 1:400), ideal peptides recovery (about 87.5%), wonderful repeatability (RSD less than 25%). Moreover, the as-prepared Fe3O4@2SiO(2)@mSiO(2)-C nanoparticles were successfully enriched 2198 endogenous peptides from human serum, which fully indicated that the mesoporous carbon nanoparticles was a promising candidate for isolating proteins or peptides from complex biologicals. |
WOS标题词 | Science & Technology ; Physical Sciences |
类目[WOS] | Chemistry, Analytical |
研究领域[WOS] | Chemistry |
关键词[WOS] | MASS-SPECTROMETRY ; FACILE PREPARATION ; STATIONARY-PHASE ; VANCOMYCIN ; SEPARATION ; NANOPARTICLES ; ENRICHMENT ; PROTEOMICS ; GRAPHENE ; ACID |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000386989500083 |
内容类型 | 期刊论文 |
源URL | [http://ir.sic.ac.cn/handle/331005/21980] |
专题 | 上海硅酸盐研究所_高性能陶瓷和超微结构国家重点实验室_期刊论文 |
作者单位 | 1.East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China 2.Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China 3.Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China |
推荐引用方式 GB/T 7714 | Gan, Yangyang,Zhang, Quanqing,Chen, Yajing,et al. Selective extraction of endogenous peptides from human serum with magnetic mesoporous carbon composites[J]. TALANTA,2016,161:647-654. |
APA | Gan, Yangyang.,Zhang, Quanqing.,Chen, Yajing.,Zhao, Yiman.,Xiong, Zhichao.,...&Zhang, Weibing.(2016).Selective extraction of endogenous peptides from human serum with magnetic mesoporous carbon composites.TALANTA,161,647-654. |
MLA | Gan, Yangyang,et al."Selective extraction of endogenous peptides from human serum with magnetic mesoporous carbon composites".TALANTA 161(2016):647-654. |
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