Role of UDP-Glucuronic Acid Decarboxylase in Xylan Biosynthesis in Arabidopsis

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	Role of UDP-Glucuronic Acid Decarboxylase in Xylan Biosynthesis in Arabidopsis
	Kuang, Beiqing 1,2; Zhao, Xianhai 1,2; Zhou, Chun 1,2; Zeng, Wei 3; Ren, Junli 4; Ebert, Berit 3; Beahan, Cherie T.3; Deng, Xiaomei 2; Zeng, Qingyin 5; Zhou, Gongke 6
刊名	MOLECULAR PLANT
	2016-08-01
卷号	9 期号:8 页码:1119-1131
关键词	UDP-Xylose xylan UDP-Glucuronic acid decarboxylase localization
英文摘要	UDP-xylose (UDP-Xyl) is the Xyl donor used in the synthesis of major plant cell-wall polysaccharides such as xylan (as a backbone-chain monosaccharide) and xyloglucan (as a branching monosaccharide). The biosynthesis of UDP-Xyl from UDP-glucuronic acid (UDP-GlcA) is irreversibly catalyzed by UDPglucuronic acid decarboxylase (UXS). Until now, little has been known about the physiological roles of UXS in plants. Here, we report that AtUXS1, AtUXS2, and AtUXS4 are located in the Golgi apparatus whereas AtUXS3, AtUXS5, and AtUXS6 are located in the cytosol. Although all six single AtUXS T-DNA mutants and the uxs1 usx2 uxs4 triple mutant show no obvious phenotype, the uxs3 uxs5 uxs6 triple mutant has an irregular xylem phenotype. Monosaccharide analysis showed that Xyl levels decreased in uxs3 uxs5 uxs6 and linkage analysis confirmed that the xylan content in uxs3 xus5 uxs6 declined, indicating that UDP-Xyl from cytosol AtUXS participates in xylan synthesis. Gel-permeation chromatography showed that the molecular weight of non-cellulosic polysaccharides in the triple mutants, mainly composed of xylans, is lower than that in the wild type, suggesting an effect on the elongation of the xylan backbone. Upon saccharification treatment stems of the uxs3 uxs5 uxs6 triple mutants released monosaccharides with a higher efficiency than those of the wild type. Taken together, our results indicate that the cytosol UXS plays a more important role than the Golgi-localized UXS in xylan biosynthesis.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Plant Sciences
研究领域[WOS]	Biochemistry & Molecular Biology ; Plant Sciences
关键词[WOS]	PLANT-CELL WALLS ; D-XYLOSE SYNTHASE ; RHAMNOGALACTURONAN-II ; MOLECULAR-CLONING ; FUNCTIONAL CLONING ; GOLGI MEMBRANES ; XYLOSYLTRANSFERASE ; EXPRESSION ; GENES ; XYLOGLUCAN
收录类别	SCI
语种	英语
WOS记录号	WOS:000386584900004
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/9075]
专题	青岛生物能源与过程研究所_微生物资源团队
作者单位	1.South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou 510642, Guangdong, Peoples R China 2.South China Agr Univ, Coll Forestry & Landscape Architecture, Guangdong Key Lab Innovat Dev & Utilizat Forest P, Guangzhou 510642, Guangdong, Peoples R China 3.Univ Melbourne, Sch BioSci, ARC Ctr Excellence Plant Cell Walls, Parkville, Vic 3010, Australia 4.South China Univ Technol, State Key Lab Pulp & Paper Engn, Guangzhou 510640, Guangdong, Peoples R China 5.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 6.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 7.Lawrence Berkeley Natl Lab, Joint BioEnergy Inst, Berkeley, CA 94720 USA 8.Lawrence Berkeley Natl Lab, Phys Biosci Div, Berkeley, CA 94720 USA 9.Guangdong Prov Res Ctr Woody Forage Engn Technol, Guangzhou 510642, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Kuang, Beiqing,Zhao, Xianhai,Zhou, Chun,et al. Role of UDP-Glucuronic Acid Decarboxylase in Xylan Biosynthesis in Arabidopsis[J]. MOLECULAR PLANT,2016,9(8):1119-1131.
APA	Kuang, Beiqing.,Zhao, Xianhai.,Zhou, Chun.,Zeng, Wei.,Ren, Junli.,...&Wu, Ai-Min.(2016).Role of UDP-Glucuronic Acid Decarboxylase in Xylan Biosynthesis in Arabidopsis.MOLECULAR PLANT,9(8),1119-1131.
MLA	Kuang, Beiqing,et al."Role of UDP-Glucuronic Acid Decarboxylase in Xylan Biosynthesis in Arabidopsis".MOLECULAR PLANT 9.8(2016):1119-1131.