Label-free, rapid and quantitative phenotyping of stress response in E. coli via ramanome

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 单细胞中心

	Label-free, rapid and quantitative phenotyping of stress response in E. coli via ramanome
	Teng, Lin 1,2,3; Wang, Xian 1,2,3; Wang, Xiaojun 1,2,3; Gou, Honglei 1,2; Ren, Lihui 1,2; Wang, Tingting 1,2; Wang, Yun 1,2; Ji, Yuetong 1,2; Huang, Wei E.4; Xu, Jian 1,2
刊名	SCIENTIFIC REPORTS
	2016-10-19
卷号	6
英文摘要	Rapid profiling of stress-response at single-cell resolution yet in a label-free, non-disruptive and mechanism-specific manner can lead to many new applications. We propose a single-cell-level biochemical fingerprinting approach named "ramanome", which is the collection of Single-cell Raman Spectra (SCRS) from a number of cells randomly selected from an isogenic population at a given time and condition, to rapidly and quantitatively detect and characterize stress responses of cellular population. SCRS of Escherichia coli cells are sensitive to both exposure time (eight time points) and dosage (six doses) of ethanol, with detection time as early as 5 min and discrimination rate of either factor over 80%. Moreover, the ramanomes upon six chemical compounds from three categories, including antibiotics of ampicillin and kanamycin, alcohols of ethanol and n-butanol and heavy metals of Cu2+ and Cr6+, were analyzed and 31 marker Raman bands were revealed which distinguish stress-responses via cytotoxicity mechanism and variation of inter-cellular heterogeneity. Furthermore, specificity, reproducibility and mechanistic basis of ramanome were validated by tracking stress-induced dynamics of metabolites and by contrasting between cells with and without genes that convey stress resistance. Thus ramanome enables rapid prediction and mechanism-based screening of cytotoxicity and stress-response programs at single-cell resolution.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	SINGLE YEAST-CELLS ; ESCHERICHIA-COLI ; MICROBIAL-CELLS ; ETHANOL STRESS ; SPECTROSCOPY ; IDENTIFICATION ; MICROSPECTROSCOPY ; COPPER ; MICROALGAE ; SIGNATURES
收录类别	SCI
语种	英语
WOS记录号	WOS:000385734500001
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/9062]
专题	青岛生物能源与过程研究所_单细胞中心
作者单位	1.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Single Cell Ctr, CAS Key Lab Biofuels, Qingdao, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Univ Oxford, Dept Engn, Parks Rd, Oxford OX1 3PJ, England
推荐引用方式 GB/T 7714	Teng, Lin,Wang, Xian,Wang, Xiaojun,et al. Label-free, rapid and quantitative phenotyping of stress response in E. coli via ramanome[J]. SCIENTIFIC REPORTS,2016,6.
APA	Teng, Lin.,Wang, Xian.,Wang, Xiaojun.,Gou, Honglei.,Ren, Lihui.,...&Xu, Jian.(2016).Label-free, rapid and quantitative phenotyping of stress response in E. coli via ramanome.SCIENTIFIC REPORTS,6.
MLA	Teng, Lin,et al."Label-free, rapid and quantitative phenotyping of stress response in E. coli via ramanome".SCIENTIFIC REPORTS 6(2016).