Folic acid deficiency inhibits neural rosette formation and neuronal differentiation from rhesus monkey embryonic stem cells

	Folic acid deficiency inhibits neural rosette formation and neuronal differentiation from rhesus monkey embryonic stem cells
	Chen YC 1,2,3,4; Wang ZB 2,5; Xie YH 1,2; Guo XY 1,2,3; Tang XH 1,2; Wang SF 1,2; Yang SH 1,2,6; Chen K 1,2; Niu YY 1,2,3; Ji WZ[*]1,2,3
刊名	JOURNAL OF NEUROSCIENCE RESEARCH
	2012
卷号	90 期号:7 页码:1382-1391
关键词	folic acid folic acid rhesus monkey embryonic stem cells neural rosette neuronal differentiation
通讯作者	wji@mail.kiz.ac.cn
英文摘要	Evidence from epidemiological studies has proved that periconceptional use of folic acid (FA) can significantly reduce the risk of neural tube defects (NTDs). However, it is hard to explore when and how FA plays roles in neurogenesis and brain development in vivo, especially in human or other nonhuman primate systems. Primate embryonic stem cell (ESC) lines are ideal models for studying cell differentiation and organogenesis in vitro. In the present study, the roles of FA in neural differentiation were assessed in a rhesus monkey ESC system in vitro. The results showed no significant difference in the expression of neural precursor markers, such as nestin, Sox-1, or Pax-6, among neural progenitors obtained from different FA concentrations or with the FA antagonist methotrexate (MTX). However, FA depletion decreased cell proliferation and affected embryoid body (EB) and neural rosette formation, as well as neuronal but not neuroglia differentiation. Our data imply that the ESC system is a suitable model for further exploring the mechanism of how FA works in prevention of NTDs in primates. (c) 2012 Wiley Periodicals, Inc.
收录类别	SCI
语种	英语
公开日期	2012-09-04
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/7014]
专题	昆明动物研究所_生殖与发育生物学昆明动物研究所_动物模型与人类重大疾病机理重点实验室昆明动物研究所_神经系统编码
作者单位	1.Laboratory of Reproductive and Developmental Biology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 2.Yunnan Key Laboratory of Animal Reproductive Biology, Kunming, China 3.Kunming Biomed International and National Engineering Research Center of Biomedicine and Animal Science, Kunming, Chi 4.Graduate School, Chinese Academy of Sciences, Beijing, China 5.Laboratory of Sensory-Motor Integration Research and Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 6.Biotechnology Research Center, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, China
推荐引用方式 GB/T 7714	Chen YC,Wang ZB,Xie YH,et al. Folic acid deficiency inhibits neural rosette formation and neuronal differentiation from rhesus monkey embryonic stem cells[J]. JOURNAL OF NEUROSCIENCE RESEARCH,2012,90(7):1382-1391.
APA	Chen YC.,Wang ZB.,Xie YH.,Guo XY.,Tang XH.,...&Ji WZ[*].(2012).Folic acid deficiency inhibits neural rosette formation and neuronal differentiation from rhesus monkey embryonic stem cells.JOURNAL OF NEUROSCIENCE RESEARCH,90(7),1382-1391.
MLA	Chen YC,et al."Folic acid deficiency inhibits neural rosette formation and neuronal differentiation from rhesus monkey embryonic stem cells".JOURNAL OF NEUROSCIENCE RESEARCH 90.7(2012):1382-1391.