CTCF interacts with the lytic HSV-1 genome to promote viral transcription

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 基因调控与表达遗传

	CTCF interacts with the lytic HSV-1 genome to promote viral transcription
	Lang FC 1,2; Li X 1,2; Xiao Y 1; Singh V 3; Lu DF 1,2; Li LH 1; Han HB 4; Wickramasinghe JM 3; Vladimirova O 3; Smith ST 5
刊名	SCIENTIFIC REPORTS
	2017
卷号	7 期号:X 页码:e39861
通讯作者	zhoujm@mail.kiz.ac.cn
英文摘要	CTCF is an essential chromatin regulator implicated in important nuclear processes including in nuclear organization and transcription. Herpes Simplex Virus-1 (HSV-1) is a ubiquitous human pathogen, which enters productive infection in human epithelial and many other cell types. CTCF is known to bind several sites in the HSV-1 genome during latency and reactivation, but its function has not been defined. Here, we report that CTCF interacts extensively with the HSV-1 DNA during lytic infection by ChIP-seq, and its knockdown results in the reduction of viral transcription, viral genome copy number and virus yield. CTCF knockdown led to increased H3K9me3 and H3K27me3, and a reduction of RNA pol II occupancy on viral genes. Importantly, ChIP-seq analysis revealed that there is a higher level of CTD Ser2P modified RNA Pol II near CTCF peaks relative to the Ser5P form in the viral genome. Consistent with this, CTCF knockdown reduced the Ser2P but increased Ser5P modified forms of RNA Pol II on viral genes. These results suggest that CTCF promotes HSV-1 lytic transcription by facilitating the elongation of RNA Pol II and preventing silenced chromatin on the viral genome.
资助信息	This work was supported by grants from Chinese Academy of Sciences (KSCXZ-EW-BR-6), Startup fund from Kunming Institute of Zoology, Chinese Academy of Sciences (Y102421081), Grants from Yunnan Provincial Government (2013FA051; 2011HA005), National Science Foundation of China (NSFC 81471966) to JZ, a NSFC grant to YX (31200964), a Common Project of Panzhihua, Science and Technology Bureau of China (2012CY-S-22(9)) to HH and a Visiting professorship for senior international scientist from CAS to NWF (2012T1S0001).
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/10790]
专题	昆明动物研究所_基因调控与表达遗传昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Kunming 650223, China 2.Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, Beijing 100101, Chin 3.Gene Expression and Regulation Program, The Wistar Institute, Philadelphia, PA 19104, USA 4.Biology & Chemistry Engineering College, Panzhihua University, Panzhihua, Sichuan 617000, China 5.Department of Biology, Arcadia University, Glenside, PA 19038, USA 6.Institutes of Biology and Medical Sciences, Soochow University, Suzhou, 215123, China 7.Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, AB T2N 4N1, Canada 8.Department of Viral Immunology, Institute of Medical Biology, Chinese Academy of Medicine Science, Peking Union Medical College, Kunming, Kunming 650118, China 9.Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
推荐引用方式 GB/T 7714	Lang FC,Li X,Xiao Y,et al. CTCF interacts with the lytic HSV-1 genome to promote viral transcription[J]. SCIENTIFIC REPORTS,2017,7(X):e39861.
APA	Lang FC.,Li X.,Xiao Y.,Singh V.,Lu DF.,...&Chen GJ.(2017).CTCF interacts with the lytic HSV-1 genome to promote viral transcription.SCIENTIFIC REPORTS,7(X),e39861.
MLA	Lang FC,et al."CTCF interacts with the lytic HSV-1 genome to promote viral transcription".SCIENTIFIC REPORTS 7.X(2017):e39861.