Migration of mouse antibody-secreting hybridoma cells from blood to genital tract and its regulation by sex hormones are associated with the differential expression patterns of adhesion molecules and chemokines in the tract rather than in the antibody-secreting cells

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	Migration of mouse antibody-secreting hybridoma cells from blood to genital tract and its regulation by sex hormones are associated with the differential expression patterns of adhesion molecules and chemokines in the tract rather than in the antibody-secreting cells
	Yu MC; Cao XM; Wang XL; Xu JJ; Yang M; Ben KL[*]
刊名	JOURNAL OF REPRODUCTIVE IMMUNOLOGY
	2007
卷号	74 期号:1-2 页码:78-89
关键词	Antibody-secreting cells Sex hormones Adhesion molecules Chemokine Genital trac
ISSN号	0165-0378
通讯作者	kben@vip.sina.com
合作状况	其它
英文摘要	To understand better the molecular mechanisms of differential migration of antibody-secreting cells (ASCs) into mouse genital tracts, and regulation by sex hormones, surface markers, hormone receptors and adhesion molecules in mouse SG2 and PA4 hybridoma cells, respectively, secreting IgG2b and polymeric IgA antibody were detected by flow cytometry or RT-PCR. Semi-quantitative RT-PCR was also used for measuring mRNA expression of adhesion molecules and chemokines (VCAM-1, ICAM-1, P-selectin, JAM-1 and CXCL12) in genital tracts of various adult mouse groups. The mRNAs of androgen receptor, estrogen receptor beta and CXCR4 were expressed in the ASCs. Sex hormones had no effect on expression of these molecules in ASCs. Except for VCAM-1, mRNA of all examined genes was expressed in normal mouse genital tracts. The mean of relative amounts of ICAM-1 and CXCL12 mRNA in all examined organs of females were higher (2.1- and 1.9-fold) than those in males. After orchiectomy or ovariectomy, the expression of ICAM-1, CXCL12 and P-selectin mRNA in the examined organs increased, except JAM-1 in male and CXCL12 in female. Sex hormone treatment recovered the changes to normal levels of mRNA expression in many examined genital tissues. In combination with our previous work, preferential migration of ASCs into female genital tract and regulation of migration by sex hormones are associated with expression patterns of adhesion molecules and chemokines in genital tract rather than in ASCs.
学科主题	Immunology ; Reproductive Biology
收录类别	SCI
资助信息	This work was supported by National Natural Science Foundation of China (30170894).
原文出处	20077478.pdf
语种	英语
公开日期	2010-08-24
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/5381]
专题	昆明动物研究所_其他
作者单位	Laboratory for Molecular and Cell Immunology, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, China
推荐引用方式 GB/T 7714	Yu MC,Cao XM,Wang XL,et al. Migration of mouse antibody-secreting hybridoma cells from blood to genital tract and its regulation by sex hormones are associated with the differential expression patterns of adhesion molecules and chemokines in the tract rather than in the antibody-secreting cells[J]. JOURNAL OF REPRODUCTIVE IMMUNOLOGY,2007,74(1-2):78-89.
APA	Yu MC,Cao XM,Wang XL,Xu JJ,Yang M,&Ben KL[*].(2007).Migration of mouse antibody-secreting hybridoma cells from blood to genital tract and its regulation by sex hormones are associated with the differential expression patterns of adhesion molecules and chemokines in the tract rather than in the antibody-secreting cells.JOURNAL OF REPRODUCTIVE IMMUNOLOGY,74(1-2),78-89.
MLA	Yu MC,et al."Migration of mouse antibody-secreting hybridoma cells from blood to genital tract and its regulation by sex hormones are associated with the differential expression patterns of adhesion molecules and chemokines in the tract rather than in the antibody-secreting cells".JOURNAL OF REPRODUCTIVE IMMUNOLOGY 74.1-2(2007):78-89.