Genomic incompatibilities in the diploid and tetraploid offspring of the goldfish × common carp cross

CORC > 昆明动物研究所 > 昆明动物研究所 > 遗传资源与进化国家重点实验室 > 分子进化基因组学

	Genomic incompatibilities in the diploid and tetraploid offspring of the goldfish × common carp cross
	Liu S[]1; Luo J 2,3; Chai J 2,3,4,5; Ren L 1; Zhou Y 1; Huang F 2,3; Liu XC 2,3; Chen YB 1; Zhang C 1; Zhang YP[]2,3,4
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2016
卷号	113 期号:5 页码:1327-1332
关键词	allopolyploidization chimeric genes sequence validation transcriptomes vertebrate
通讯作者	lsj@hunnu.edu.cn
合作状况	其它
英文摘要	Polyploidy is much rarer in animals than in plants but it is not known why. The outcome of combining two genomes in vertebrates remains unpredictable, especially because polyploidization seldom shows positive effects and more often results in lethal consequences because viable gametes fail to form during meiosis. Fortunately, the goldfish (maternal) × common carp (paternal) hybrids have reproduced successfully up to generation 22, and this hybrid lineage permits an investigation into the genomics of hybridization and tetraploidization. The first two generations of these hybrids are diploids, and subsequent generations are tetraploids. Liver transcriptomes from four generations and their progenitors reveal chimeric genes (>9%) and mutations of orthologous genes. Characterizations of 18 randomly chosen genes from genomic DNA and cDNA confirm the chimera. Some of the chimeric and differentially expressed genes relate to mutagenesis, repair, and cancer-related pathways in 2nF₁. Erroneous DNA excision between homologous parental genes may drive the high percentage of chimeric genes, or even more potential mechanisms may result in this phenomenon. Meanwhile, diploid offspring show paternal-biased expression, yet tetraploids show maternal-biased expression. These discoveries reveal that fast and unstable changes are mainly deleterious at the level of transcriptomes although some offspring still survive their genomic abnormalities. In addition, the synthetic effect of genome shock might have resulted in greatly reduced viability of 2nF₂ hybrid offspring. The goldfish × common carp hybrids constitute an ideal system for unveiling the consequences of intergenomic interactions in hybrid vertebrate genomes and their fertility.
收录类别	SCI
资助信息	This research was supported by National Natural Science Foundation of China Grants 30930071, 91331105, 31360514, 31430088, and 31210103918, the Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province (20134486), the Construction Project of Key Discipline of Hunan Province and China, the State Key Laboratory of Genetics, Resources and Evolution, Kunming Institute of Zoology, CAS, Strategic Priority Research Program of the Chinese Academy of Sciences (Grant XDB13040300), and the Program for Innovative Research Team (in Science and Technology) in the University of Yunnan Province.
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/9531]
专题	昆明动物研究所_分子进化基因组学昆明动物研究所_遗传资源与进化国家重点实验室
作者单位	1.Key Laboratory of Protein Chemistry and Fish Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, 410081, Hunan, China 2.State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, 650091, Yunnan, China 3.Key Laboratory for Animal Genetic Diversity and Evolution of High Education in Yunnan Province, School of Life Sciences, Yunnan University, Kunming, 650091, Yunnan, China 4.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, Yunnan, China 5.Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, 650204, China 6.School of Software, Yunnan University, Kunming, 650091, Yunnan, China 7.Core Genomic Facility, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100029, China 8.Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100101, China 9.Centre for Biodiversity and Conservation Biology, Royal Ontario Museum, Toronto, ON, Canada M5S 2C6 10.j Department of Biology, University of Konstanz, 78457 Konstanz, Germany
推荐引用方式 GB/T 7714	Liu S[*],Luo J,Chai J,et al. Genomic incompatibilities in the diploid and tetraploid offspring of the goldfish × common carp cross[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2016,113(5):1327-1332.
APA	Liu S[].,Luo J.,Chai J.,Ren L.,Zhou Y.,...&Zhang YP[].(2016).Genomic incompatibilities in the diploid and tetraploid offspring of the goldfish × common carp cross.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,113(5),1327-1332.
MLA	Liu S[*],et al."Genomic incompatibilities in the diploid and tetraploid offspring of the goldfish × common carp cross".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 113.5(2016):1327-1332.