Effects of Pallas' viper (Agkistrodon halys pallas) venom on blood coagulation and characterization of a prothrombin activator

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 动物活性蛋白多肽组学

	Effects of Pallas' viper (Agkistrodon halys pallas) venom on blood coagulation and characterization of a prothrombin activator
	Zhang Y; Lee WH; Gao R; Xiong YL; Wang WY; Zhu SW
刊名	TOXICON
	1998
卷号	36 期号:1 页码:143-152
ISSN号	0041-0101
合作状况	其它
英文摘要	The action of Pallas' viper (Agkistrodon halys pallas) venom on blood coagulation was examined in vitro and a strong anticoagulant effect was observed. This action was abolished after treatment with a specific inhibitor of phospholipase A2 activity (p-bromophenacyl bromide), revealing a procoagulant action in low concentrations of treated venom (around 1 microgram/ml). The effect of the venom on haemostasis was further characterized by measuring its ability to activate purified blood coagulation factors. It is concluded that A. halys pallas venom contains prothrombin activation activity. A prothrombin activator (aharin) was purified from the venom by Sephadex G-75 gel filtration and ion-exchange chromatography on a Mono-Q column. It consisted of a single polypeptide chain, with a mol. wt of 63,000. Purified aharin possessed no amidolytic activity on chromogenic substrates. It did not act on other blood coagulation factors, such as factor X and plasminogen, nor did it cleave or clot purified fibrinogen. The prothrombin activation activity of aharin was readily inhibited by ethylenediamine tetracetic acid (a metal chelator), but specific serine protease inhibitors such as diisopropyl fluorophosphate and phenylmethanesulfonyl fluoride had no effect on it. These observations suggest that, like those prothrombin activators from Echis carinatus and Bothrops atrox venoms, the prothrombin activator from A. halys pallas venom is a metalloproteinase.
收录类别	SCI
资助信息	This study was supported in part by grants from the National Natural Science Foundation (39740001) and Yunnan Science and Technology Commission (96C002R), and in part by a grant from the Chinese Academy of Sciences for young scientists in ‘9th five-year plan’.
原文出处	199836143.pdf
语种	英语
公开日期	2010-08-24
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/2633]
专题	昆明动物研究所_动物活性蛋白多肽组学昆明动物研究所_其他
作者单位	Department of Animal Toxinology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, P.R. China
推荐引用方式 GB/T 7714	Zhang Y,Lee WH,Gao R,et al. Effects of Pallas' viper (Agkistrodon halys pallas) venom on blood coagulation and characterization of a prothrombin activator[J]. TOXICON,1998,36(1):143-152.
APA	Zhang Y,Lee WH,Gao R,Xiong YL,Wang WY,&Zhu SW.(1998).Effects of Pallas' viper (Agkistrodon halys pallas) venom on blood coagulation and characterization of a prothrombin activator.TOXICON,36(1),143-152.
MLA	Zhang Y,et al."Effects of Pallas' viper (Agkistrodon halys pallas) venom on blood coagulation and characterization of a prothrombin activator".TOXICON 36.1(1998):143-152.