Purification and characterization of cholecystokinin from the skin of salamander Tylototriton verrucosus | |
Jiang WB1; HAKIM M2; Luo L2; Li BW2; Yang SL2; Song YZ[*]1; Lai R2; Lu QM[*]2 | |
刊名 | Zoological research |
2015 | |
卷号 | 36期号:3页码:174-177 |
关键词 | Cholecystokinin Salamander Skin Amphibian |
通讯作者 | lvqm@mail.kiz.ac.cn ; yuzhusong@ kmust.edu.cn |
合作状况 | 其它 |
英文摘要 | As a group of intestinal hormones and neurotransmitters, cholecystokinins (CCKs) regulate and affect pancreatic enzyme secretion, gastrointestinal motility, pain hypersensitivity, digestion and satiety, and generally contain a DYMGWMDFG sequence at the C-terminus. Many CCKs have been reported in mammals. However, only a few have been reported in amphibians, such as Hyla nigrovittata, Xenopus laevis, and Rana catesbeiana, with none reported in urodele amphibians like newts and salamanders. Here, a CCK called CCK-TV was identified and characterized from the skin of the salamander Tylototriton verrucosus. This CCK contained an amino acid sequence of DYMGWMDF-NH2 as seen in other CCKs. A cDNA encoding the CCK precursor containing 129 amino acid residues was cloned from the cDNA library of T. verrucosus skin. The CCK-TV had the potential to induce the contraction of smooth muscle strips isolated from porcine gallbladder, eliciting contraction at a concentration of 5.0x10-11 mol/L and inducing maximal contraction at a concentration of 2.0x10-6 mol/L. The EC50 was 13.6 nmol/L. To the best of our knowledge, this is the first report to identify the presence of a CCK in an urodele amphibian. |
收录类别 | 其他 |
资助信息 | This work was supported by the National Basic Research Program of China (973 Program) (2013CB911300), National Natural Science Foundation of China (U1132601) and the Chinese Academy of Sciences (SAJC201308) |
语种 | 英语 |
公开日期 | 2015-06-08 |
内容类型 | 期刊论文 |
源URL | [http://159.226.149.42:8088/handle/152453/8337] |
专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
作者单位 | 1.Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming Yunnan 650500, China; 2.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming Yunnan 650223, China |
推荐引用方式 GB/T 7714 | Jiang WB,HAKIM M,Luo L,et al. Purification and characterization of cholecystokinin from the skin of salamander Tylototriton verrucosus[J]. Zoological research,2015,36(3):174-177. |
APA | Jiang WB.,HAKIM M.,Luo L.,Li BW.,Yang SL.,...&Lu QM[*].(2015).Purification and characterization of cholecystokinin from the skin of salamander Tylototriton verrucosus.Zoological research,36(3),174-177. |
MLA | Jiang WB,et al."Purification and characterization of cholecystokinin from the skin of salamander Tylototriton verrucosus".Zoological research 36.3(2015):174-177. |
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