Purification, cloning and biological characterization of a novel disintegrin from Trimeresurus jerdonii venom

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	Purification, cloning and biological characterization of a novel disintegrin from Trimeresurus jerdonii venom
	Zhou XD 1,2,3; Jin Y 1; Chen RQ 1,2; Lu QM 1; Wu JB 1,2; Wang WY 1; Xiong YL[*]1
刊名	TOXICON
	2004
卷号	43 期号:1 页码:69-75
关键词	Snake venom Tumor Disintegrin Platelet
ISSN号	0041-0101
通讯作者	zhouxingding@vip.sina.com
合作状况	其它
英文摘要	A novel disintegrin, jerdonin, was purified from the Trimeresurus jerdonii venom by means of gel filtration and reverse phase high pressure liquid chromatography. Its coding cDNA was also isolated from the venom gland. The jerdonin coding cDNA is part of a precursor composed of proprotein, metalloproteinase, and disintegrin domains. From the deduced amino acid sequence, jerdonin is composed of 71 amino acid residues including 12 cysteines and the tripeptide sequence Arg-Gly-Asp (RGD), a well-known characteristic of the disintegrin family. Molecular mass of jerdonin was determined to be 7483Da by matrix-assisted laser desorption ionization time of flight mass spectrometry. Jerdonin inhibited ADP- and collagen-induced human platelet aggregation with IC(50) of 220 and 240 nM, respectively. In vivo, jerdonin inhibited the growth of subcutaneously inoculated B16 solid tumor in C57BL/6 mice and improved the survival time of the tumor-bearing mice.
收录类别	SCI
资助信息	This research was supported with fund from Natural Science Foundation of Yunnan Science and Technology Committee, No. 2002C0063M.
原文出处	20044369.pdf
语种	英语
公开日期	2010-08-24
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/4135]
专题	昆明动物研究所_动物毒素室昆明动物研究所_其他
作者单位	1.Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223 Yunnan, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100080, China 3.Faculty of Biological and Chemical Engineering, Kumming University of Science and Technology, 650224 Kunming, Yunnan, China
推荐引用方式 GB/T 7714	Zhou XD,Jin Y,Chen RQ,et al. Purification, cloning and biological characterization of a novel disintegrin from Trimeresurus jerdonii venom[J]. TOXICON,2004,43(1):69-75.
APA	Zhou XD.,Jin Y.,Chen RQ.,Lu QM.,Wu JB.,...&Xiong YL[*].(2004).Purification, cloning and biological characterization of a novel disintegrin from Trimeresurus jerdonii venom.TOXICON,43(1),69-75.
MLA	Zhou XD,et al."Purification, cloning and biological characterization of a novel disintegrin from Trimeresurus jerdonii venom".TOXICON 43.1(2004):69-75.