Establishment and characterization of a fibroblast-like cell line from Anabarilius grahami (Cypriniformes: Cyprinidae)

CORC > 昆明动物研究所 > 昆明动物研究所 > 遗传资源与进化国家重点实验室 > 系统进化与生物地理学

	Establishment and characterization of a fibroblast-like cell line from Anabarilius grahami (Cypriniformes: Cyprinidae)
	Wang XA 1,2; Yang JX[]1; Chen XY[]1; Pan XF 1
刊名	Zoological Research
	2012
卷号	33 期号:E5−6 页码:E89−E97
关键词	Characterization Fibroblast-like cell line Anabarilius grahami Cryopreservation
通讯作者	yangjx@mail.kiz.ac.cn ; chenxy@mail.kiz.ac.cn
合作状况	其它
英文摘要	Though Yunnan province contains some 562 known species of fish, no cell lines from any of these have been made available to date. To protect germplasm resources and provide an effective tool in solving problems at cellular level of Anabarilius grahami, a fish endemic to Fuxian Lake, Yunnan, China, we established and characterized the major features of a continuous cell line (AGF II) from the caudal fin tissue of A. grahami. This AGF II cell line consists of fibroblast-like cells and has been subcultured more than 60 times over the course of a year. The cell line was maintained in DMEM/F12 supplemented with 10% FBS, with a cellular doubling time of 51.1 h. We continued with more experiments to optimize the culture and storage conditions, and found a variety of interesting results: cells could grow at temperature between 24 °C and 28 °C, with the optimal temperature of 28 °C. Likewise, the growth rate of A. grahami fin cells increased when the FBS proportion increased from 5% to 20%, with the optimal growth at the concentrations of 20% FBS; cells were able to grow in L-15 and DMEM/F12 with optimal growth at L-15; DMSO is a better cryoprotectant than Glycerol, EG and MeOH for AGFII cells with optimal concentration of 5% DMSO. Chromosome analysis also showed that the distribution of chromosome number varies from 38 to 52, with a modal peak at 48 chromosomes, accounting for 39.8% of all cells. Using the same primer pairs specific to mtDNA, the AGF II cell sequences obtained by PCR were identical to those from muscle tissues of A. grahami. Both chromosome analysis and PCR amplification confirmed the AGF II cells were from A. grahami, also indicating that that current long-term artificial propagation of A. grahami has been successful. Finally, we noted that when cells were transfected with pEYFP-N1 and pECFP-N1 plasmid, bright fluorescent signals were observed, suggesting that this cell line may be suitable for use in transfection and future gene expression studies.
收录类别	其他
资助信息	This work was supported by the Global Environment Foundation/The World Bank Project (GEF-MSP grant No. TF051795); the Yunnan Development and Reform Commission
语种	英语
公开日期	2013-09-17
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/7639]
专题	昆明动物研究所_系统进化与生物地理学昆明动物研究所_遗传资源与进化国家重点实验室昆明动物研究所_东南亚野生动物多样性
作者单位	1.State key laboratory of genetic resources and evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.University of the Chinese Academy of Sciences, Beijing 100049, China
推荐引用方式 GB/T 7714	Wang XA,Yang JX[],Chen XY[],et al. Establishment and characterization of a fibroblast-like cell line from Anabarilius grahami (Cypriniformes: Cyprinidae)[J]. Zoological Research,2012,33(E5−6):E89−E97.
APA	Wang XA,Yang JX[],Chen XY[],&Pan XF.(2012).Establishment and characterization of a fibroblast-like cell line from Anabarilius grahami (Cypriniformes: Cyprinidae).Zoological Research,33(E5−6),E89−E97.
MLA	Wang XA,et al."Establishment and characterization of a fibroblast-like cell line from Anabarilius grahami (Cypriniformes: Cyprinidae)".Zoological Research 33.E5−6(2012):E89−E97.